{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:17:57Z","timestamp":1791177477905,"version":"4.1.0"},"reference-count":48,"publisher":"National Academy of Sciences","issue":"7","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2010,2,16]]},"abstract":"<jats:p>\n                    Cholera is an acute intestinal infection caused by the bacterium\n                    <jats:italic>Vibrio cholerae<\/jats:italic>\n                    . In order for\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    to cause disease, it must produce two virulence factors, the toxin-coregulated pilus (TCP) and cholera toxin (CT), whose expression is controlled by a transcriptional cascade culminating with the expression of the AraC-family regulator, ToxT. We have solved the 1.9\u00a0\u212b resolution crystal structure of ToxT, which reveals folds in the N- and C-terminal domains that share a number of features in common with AraC, MarA, and Rob as well as the unexpected presence of a buried 16-carbon fatty acid,\n                    <jats:italic>cis<\/jats:italic>\n                    -palmitoleate. The finding that\n                    <jats:italic>cis<\/jats:italic>\n                    -palmitoleic acid reduces TCP and CT expression in\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    and prevents ToxT from binding to DNA in vitro provides a direct link between the host environment of\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    and regulation of virulence gene expression.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0915021107","type":"journal-article","created":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T22:51:30Z","timestamp":1265064690000},"page":"2860-2865","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":162,"title":["Structure of\n                    <i>Vibrio cholerae<\/i>\n                    ToxT reveals a mechanism for fatty acid regulation of virulence genes"],"prefix":"10.1073","volume":"107","author":[{"given":"Michael J.","family":"Lowden","sequence":"first","affiliation":[{"name":"Department of Chemistry, Dartmouth College, 6128 Burke Laboratory, Hanover, NH 03755; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Skorupski","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Dartmouth Medical School, Vail Building Room 106, Hanover, NH 03755"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Pellegrini","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Dartmouth College, 6128 Burke Laboratory, Hanover, NH 03755; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael G.","family":"Chiorazzo","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Dartmouth College, 6128 Burke Laboratory, Hanover, NH 03755; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald K.","family":"Taylor","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Dartmouth Medical School, Vail Building Room 106, Hanover, NH 03755"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. 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