{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:04:28Z","timestamp":1791263068848,"version":"4.1.0"},"reference-count":37,"publisher":"National Academy of Sciences","issue":"8","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2010,2,23]]},"abstract":"<jats:p>\n                    GNA2132 is a\n                    <jats:italic>Neisseria meningitidis<\/jats:italic>\n                    antigen of unknown function, discovered by reverse vaccinology, which has been shown to induce bactericidal antibodies in animal models. Here we show that this antigen induces protective immunity in humans and it is recognized by sera of patients after meningococcal disease. The protein binds heparin in vitro through an Arg-rich region and this property correlates with increased survival of the unencapsulated bacterium in human serum. Furthermore, two proteases, the meningococcal NalP and human lactoferrin, cleave the protein upstream and downstream from the Arg-rich region, respectively. We conclude that GNA2132 is an important protective antigen of\n                    <jats:italic>N. meningitidis<\/jats:italic>\n                    and we propose to rename it,\n                    <jats:underline>N<\/jats:underline>\n                    eisserial\n                    <jats:underline>H<\/jats:underline>\n                    eparin\n                    <jats:underline>B<\/jats:underline>\n                    inding\n                    <jats:underline>A<\/jats:underline>\n                    ntigen (NHBA).\n                  <\/jats:p>","DOI":"10.1073\/pnas.0915162107","type":"journal-article","created":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T22:10:32Z","timestamp":1265235032000},"page":"3770-3775","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":187,"title":["<i>Neisseria meningitidis<\/i>\n                    GNA2132, a heparin-binding protein that induces protective immunity in humans"],"prefix":"10.1073","volume":"107","author":[{"given":"Davide","family":"Serruto","sequence":"first","affiliation":[{"name":"Novartis Vaccines and Diagnostics, 53100 Siena, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiziana","family":"Spadafina","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, 53100 Siena, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Ciucchi","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, 53100 Siena, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa A.","family":"Lewis","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, MA 01655;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjay","family":"Ram","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, MA 01655;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Tontini","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, 53100 Siena, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Santini","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, 53100 Siena, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessia","family":"Biolchi","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, 53100 Siena, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kate L.","family":"Seib","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, 53100 Siena, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marzia M.","family":"Giuliani","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, 53100 Siena, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John J.","family":"Donnelly","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, 53100 Siena, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Berti","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, 53100 Siena, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvana","family":"Savino","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, 53100 Siena, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Scarselli","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, 53100 Siena, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Costantino","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, 53100 Siena, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Simon","family":"Kroll","sequence":"additional","affiliation":[{"name":"Department of Paediatrics, Faculty of Medicine, Imperial College London, London, W2 1PG United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00edona","family":"O\u2019Dwyer","sequence":"additional","affiliation":[{"name":"Department of Paediatrics, Faculty of Medicine, Imperial College London, London, W2 1PG United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiazhou","family":"Qiu","sequence":"additional","affiliation":[{"name":"Gastroenterology Division, Department of Medicine and the Clinical Translational Research Institute, Tufts Medical Center, Boston, MA 02111; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew G.","family":"Plaut","sequence":"additional","affiliation":[{"name":"Gastroenterology Division, Department of Medicine and the Clinical Translational Research Institute, Tufts Medical Center, Boston, MA 02111; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Moxon","sequence":"additional","affiliation":[{"name":"Medical Sciences Division, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rino","family":"Rappuoli","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, 53100 Siena, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariagrazia","family":"Pizza","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, 53100 Siena, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatrice","family":"Aric\u00f2","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, 53100 Siena, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2010,2,23]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)61016-2"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.03.091"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2006.00051.x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5459.1816"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0603940103"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/379375"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00036-08"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00007-09"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.1.1-8.1997"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.12.7566"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.1.501"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.5.1790-1795.1995"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.8.4447-4461.2002"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03773.x"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03327.x"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.21.12641"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.02.098"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2008.12.052"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.11.006"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00936-09"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1039\/b100916h"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1991.71.2.481"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00763.x"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.184.3.993"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.11.5010-5019.1994"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(92)90112-W"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.9.4463-4468.1999"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02841.x"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-6-1813"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.5.1068"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1996.tb02641.x"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1078\/1438-4221-00142"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)55379-5"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(93)90079-Q"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2005.08.003"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(09)70132-X"},{"key":"e_1_3_3_37_2","first-page":"970","article-title":"Interlaboratory standardization of the measurement of serum bactericidal activity by using human complement against meningococcal serogroup b, strain 44\/76-SL, before and after vaccination with the Norwegian MenBvac outer membrane vesicle vaccine","volume":"12","author":"Borrow R","year":"2005","unstructured":"R Borrow, et al., Interlaboratory standardization of the measurement of serum bactericidal activity by using human complement against meningococcal serogroup b, strain 44\/76-SL, before and after vaccination with the Norwegian MenBvac outer membrane vesicle vaccine. Clin Diagn Lab Immunol 12, 970\u2013976 (2005).","journal-title":"Clin Diagn Lab Immunol"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.0915162107","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T18:58:51Z","timestamp":1649789931000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.0915162107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,2,23]]},"references-count":37,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2010,2,23]]}},"alternative-id":["10.1073\/pnas.0915162107"],"URL":"https:\/\/doi.org\/10.1073\/pnas.0915162107","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,2,23]]},"assertion":[{"value":"2010-02-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}