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Intracellular survival of\n                    <jats:italic>Salmonella<\/jats:italic>\n                    involves segregation in an endomembrane compartment (\n                    <jats:italic>Salmonella<\/jats:italic>\n                    -containing vacuole, SCV) that remains negative for the mannose-6-phosphate receptor (M6PR) and that is inaccessible to the endocytic pathway. Expression of Nramp1 at the membrane of SCVs stimulates both acquisition of M6PR and accessibility to newly formed endosomes. The possible role of Nramp1-mediated iron transport on SCV maturation was investigated with membrane-permeant iron chelators. Pretreatment of primary macrophages from\n                    <jats:italic>Nramp1<\/jats:italic>\n                    mutant mice or of RAW264.7 macrophages (from BALB\/c mice bearing an\n                    <jats:italic>\n                      Nramp1\n                      <jats:sup>D169<\/jats:sup>\n                    <\/jats:italic>\n                    -deficient allele) with either desferrioxamine or salicylaldehyde isocotinoyl hydrazone restored recruitment of M6PR and delivery of the fluid phase marker rhodamine dextran to SCVs to levels similar to those seen in macrophages expressing WT\n                    <jats:italic>Nramp1<\/jats:italic>\n                    . The effect was specific and dose-dependent and could be abrogated by preincubation with excess iron. These data suggest that Nramp1-mediated deprivation of iron and possibly of other divalent metals in macrophages antagonizes the ability of\n                    <jats:italic>Salmonella<\/jats:italic>\n                    to alter phagosome maturation.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0937287100","type":"journal-article","created":{"date-parts":[[2003,5,13]],"date-time":"2003-05-13T14:28:41Z","timestamp":1052836121000},"page":"6127-6132","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":37,"title":["Iron chelators modulate the fusogenic properties of\n                    <i>Salmonella<\/i>\n                    -containing phagosomes"],"prefix":"10.1073","volume":"100","author":[{"given":"Nada","family":"Jabado","sequence":"first","affiliation":[{"name":"Department of Biochemistry, McGill University, Montreal, QC, Canada H3G-1Y6 North America; and Division of Cellular Biology, Hospital for Sick Children, Toronto, ON, Canada M5G-1X8 North America"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Cuellar-Mata","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, McGill University, Montreal, QC, Canada H3G-1Y6 North America; and Division of Cellular Biology, Hospital for Sick Children, Toronto, ON, Canada M5G-1X8 North America"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Grinstein","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, McGill University, Montreal, QC, Canada H3G-1Y6 North America; and Division of Cellular Biology, Hospital for Sick Children, Toronto, ON, Canada M5G-1X8 North America"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Gros","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, McGill University, Montreal, QC, Canada H3G-1Y6 North America; and Division of Cellular Biology, Hospital for Sick Children, Toronto, ON, Canada M5G-1X8 North America"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2003,4,23]]},"reference":[{"key":"e_1_3_3_1_2","first-page":"407","volume-title":"Immunology of Infectious Diseases","author":"Gros P","year":"2002","unstructured":"P Gros, E Schurr Immunology of Infectious Diseases, eds S H E Kaufman, A Sher, R Ahmed (Am. 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