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Two populations of PA pores were visualized in membranes, vesicle-inserted and nanodisc-inserted, allowing us to reconstruct two virtually identical PA-pore structures at 22-\u212b resolution. Reconstruction of a domain 4-truncated PA pore inserted into nanodiscs showed that this domain does not significantly influence pore structure. Normal mode flexible fitting of the x-ray crystallographic coordinates of the PA prepore indicated that a prominent flange observed within the pore lumen is formed by the convergence of mobile loops carrying Phe427, a residue known to catalyze protein translocation. Our results have identified the location of a crucial functional element of the PA pore and documented the value of combining nanodisc technology with electron microscopy to examine the structures of membrane-interactive proteins.<\/jats:p>","DOI":"10.1073\/pnas.1000100107","type":"journal-article","created":{"date-parts":[[2010,2,8]],"date-time":"2010-02-08T21:53:28Z","timestamp":1265666008000},"page":"3453-3457","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":92,"title":["Three-dimensional structure of the anthrax toxin pore inserted into lipid nanodiscs and lipid vesicles"],"prefix":"10.1073","volume":"107","author":[{"given":"H.","family":"Katayama","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Harvard Medical School, Alpert Building, Boston, MA 02115;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Tama","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of Arizona, Tucson, AZ 85721-0088"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Chollet","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. 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