{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T06:44:50Z","timestamp":1792737890013,"version":"4.5.0"},"reference-count":40,"publisher":"National Academy of Sciences","issue":"10","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2010,3,9]]},"abstract":"<jats:p>\n                    Dengue and dengue hemorrhagic fever are increasing public health problems with an estimated 50\u2013100\u00a0million new infections each year.\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    is the major vector of dengue viruses in its range and control of this mosquito would reduce significantly human morbidity and mortality. Present mosquito control methods are not sufficiently effective and new approaches are needed urgently. A \u201csterile-male-release\u201d strategy based on the release of mosquitoes carrying a conditional dominant lethal gene is an attractive new control methodology. Transgenic strains of\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    were engineered to have a repressible female-specific flightless phenotype using either two separate transgenes or a single transgene, based on the use of a female-specific indirect flight muscle promoter from the\n                    <jats:italic>Aedes aegypti Actin-4<\/jats:italic>\n                    gene. These strains eliminate the need for sterilization by irradiation, permit male-only release (\u201cgenetic sexing\u201d), and enable the release of eggs instead of adults. Furthermore, these strains are expected to facilitate area-wide control or elimination of dengue if adopted as part of an integrated pest management strategy.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1000251107","type":"journal-article","created":{"date-parts":[[2010,2,22]],"date-time":"2010-02-22T22:35:31Z","timestamp":1266878131000},"page":"4550-4554","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":306,"title":["Female-specific flightless phenotype for mosquito control"],"prefix":"10.1073","volume":"107","author":[{"given":"Guoliang","family":"Fu","sequence":"first","affiliation":[{"name":"Oxitec Limited, 71 Milton Park, Oxford OX14 4RX, United Kingdom;"},{"name":"Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosemary S.","family":"Lees","sequence":"additional","affiliation":[{"name":"Oxitec Limited, 71 Milton Park, Oxford OX14 4RX, United Kingdom;"},{"name":"Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derric","family":"Nimmo","sequence":"additional","affiliation":[{"name":"Oxitec Limited, 71 Milton Park, Oxford OX14 4RX, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane","family":"Aw","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697-3900; 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