{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T06:58:43Z","timestamp":1792738723583,"version":"4.5.0"},"reference-count":54,"publisher":"National Academy of Sciences","issue":"16","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2010,4,20]]},"abstract":"<jats:p>\n                    The spirochetal agent of Lyme disease,\n                    <jats:italic>Borrelia burgdorferi<\/jats:italic>\n                    , is transmitted by bites of\n                    <jats:italic>Ixodes<\/jats:italic>\n                    ticks to mammalian reservoir hosts and humans. The mechanism(s) by which the organism is trafficked from vector to host is poorly understood. In this study, we demonstrate that a\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    mutant strain deficient in the synthesis of the\n                    <jats:italic>bba64<\/jats:italic>\n                    gene product was incapable of infecting mice via tick bite even though the mutant was (\n                    <jats:italic>i<\/jats:italic>\n                    ) infectious in mice when introduced by needle inoculation, (\n                    <jats:italic>ii<\/jats:italic>\n                    ) acquired by larval ticks feeding on infected mice, and (\n                    <jats:italic>iii<\/jats:italic>\n                    ) able to persist through tick molting stages. This finding of a\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    gene required for pathogen transfer and\/or survival from the tick to the susceptible host represents an important breakthrough toward understanding transmission mechanisms involved for the Lyme disease agent.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1000268107","type":"journal-article","created":{"date-parts":[[2010,4,5]],"date-time":"2010-04-05T20:35:31Z","timestamp":1270499731000},"page":"7515-7520","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":62,"title":["The\n                    <i>bba64<\/i>\n                    gene of\n                    <i>Borrelia burgdorferi<\/i>\n                    , the Lyme disease agent, is critical for mammalian infection via tick bite transmission"],"prefix":"10.1073","volume":"107","author":[{"suffix":"Jr.","given":"Robert 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