{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:48:45Z","timestamp":1792597725284,"version":"4.5.0"},"reference-count":42,"publisher":"National Academy of Sciences","issue":"25","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2010,6,22]]},"abstract":"<jats:p>\n                    To investigate the possibility of using commensal bacteria as signal mediators for inhibiting the disease cholera, we stably transformed\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    Nissle 1917 (Nissle) to express the autoinducer molecule cholera autoinducer 1 (CAI-1) (shown previously to prevent virulence when present with another signaling molecule, autoinducer 2, at high concentrations) and determined the effect on\n                    <jats:italic>Vibrio cholerae<\/jats:italic>\n                    virulence gene expression and colonization in an infant mouse model. We found that pretreatment of mice for 8\u00a0h with Nissle engineered to express CAI-1 (Nissle-cqsA) greatly increased the mice\u2019s survival (92%) from ingestion of\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    . Pretreatment with Nissle-cqsA for only 4\u00a0h increased survival by 77%, whereas ingesting Nissle-cqsA at the same time as\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    increased survival rates by 27%. Immunostaining revealed an 80% reduction in cholera toxin binding to the intestines of mice pretreated for 8\u00a0h with Nissle-cqsA. Further, the numbers of\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    in treated mouse intestines was reduced by 69% after 40\u00a0h. This finding points to an easily administered and inexpensive approach where commensal bacteria are engineered to communicate with invasive species and potentially prevent human disease.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1001294107","type":"journal-article","created":{"date-parts":[[2010,6,7]],"date-time":"2010-06-07T20:17:25Z","timestamp":1275941845000},"page":"11260-11264","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":230,"title":["Engineered bacterial communication prevents\n                    <i>Vibrio cholerae<\/i>\n                    virulence in an infant mouse model"],"prefix":"10.1073","volume":"107","author":[{"given":"Faping","family":"Duan","sequence":"first","affiliation":[{"name":"Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John C.","family":"March","sequence":"additional","affiliation":[{"name":"Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2010,6,7]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1110591"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1499"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/78.4.675"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1080\/10408410902733946"},{"key":"e_1_3_3_5_2","first-page":"17","article-title":"Interactions of intestinal epithelial cells with bacteria and immune cells: Methods to characterize microflora and functional consequences","volume":"341","author":"Canny G","year":"2006","unstructured":"G Canny, A Swidsinski, BA McCormick, Interactions of intestinal epithelial cells with bacteria and immune cells: Methods to characterize microflora and functional consequences. 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