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An identical hypertrophic growth response was measured in CMs from CTR mice and \u03b2RM and in isolated adult CMs cultured with or without 1 \u03bcM ISO. In both genotypes, ISO infusion induced similar changes in the expression of hypertrophy-associated cardiac genes and significant elevation of serum atrial natriuretic peptide and total cardiac cGMP. No differences in cardiac hypertrophy were obtained by 7-day ISO infusion in 4- to 6-week-old conventional cGKI-KO and CTR mice. Furthermore, TAC-induced hypertrophy of CTR mice and \u03b2RM was not different and did not result in changes of the cGMP-hydrolyzing phosphodiesterase activities in hypertropic hearts or CMs. These results strongly suggest that cardiac myocyte cGKI does not affect the development of heart hypertrophy induced by pressure overload or chronic ISO infusion.<\/jats:p>","DOI":"10.1073\/pnas.1001360107","type":"journal-article","created":{"date-parts":[[2010,3,9]],"date-time":"2010-03-09T00:29:02Z","timestamp":1268094542000},"page":"5646-5651","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":98,"title":["Cardiac hypertrophy is not amplified by deletion of cGMP-dependent protein kinase I in cardiomyocytes"],"prefix":"10.1073","volume":"107","author":[{"given":"Robert","family":"Lukowski","sequence":"first","affiliation":[{"name":"Forschergruppe 923 and"},{"name":"Institut f\u00fcr Pharmakologie und Toxikologie, Technische Universit\u00e4t M\u00fcnchen, D-80802 Munich, Germany;"},{"name":"Department of Pharmacology, Toxicology, and Clinical Pharmacy, Institute of Pharmacy, University of T\u00fcbingen, D-72076 Tuebingen, Germany; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergei D.","family":"Rybalkin","sequence":"additional","affiliation":[{"name":"Forschergruppe 923 and"},{"name":"Center for Integrated Protein Science Munich, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, D-81377 Munich, Germany;"},{"name":"Department of Pharmacology, University of Washington, Seattle, WA 98195-7280"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Loga","sequence":"additional","affiliation":[{"name":"Forschergruppe 923 and"},{"name":"Center for Integrated Protein Science Munich, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, D-81377 Munich, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronika","family":"Leiss","sequence":"additional","affiliation":[{"name":"Forschergruppe 923 and"},{"name":"Center for Integrated Protein Science Munich, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, D-81377 Munich, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph A.","family":"Beavo","sequence":"additional","affiliation":[{"name":"Forschergruppe 923 and"},{"name":"Center for Integrated Protein Science Munich, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, D-81377 Munich, Germany;"},{"name":"Department of Pharmacology, University of Washington, Seattle, WA 98195-7280"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franz","family":"Hofmann","sequence":"additional","affiliation":[{"name":"Forschergruppe 923 and"},{"name":"Center for Integrated Protein Science Munich, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, D-81377 Munich, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2010,3,8]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2005.10.001"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.26.14730"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/378065a0"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI11273"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.051625598"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI17061"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119883"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.35.1.19"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1677\/jme.1.02138"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7839143"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000144801.09557.4c"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2003.12.005"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0510019103"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.107.157677"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703679"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.45.120403.095802"},{"key":"e_1_3_3_17_2","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1016\/S0022-3565(24)35197-3","article-title":"Analysis of expression of cGMP-dependent protein kinase in rabbit heart cells","volume":"291","author":"Kumar R","year":"1999","unstructured":"R Kumar, RW Joyner, P Komalavilas, TM Lincoln, Analysis of expression of cGMP-dependent protein kinase in rabbit heart cells. 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