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On cell lines mixtures, we demonstrated a capture yield better than 94%, and the possibility to cultivate in situ the captured cells. A second series of experiments involved clinical samples\u2014blood, pleural effusion, and fine needle aspirates\u2014 issued from healthy donors and patients with B-cell hematological malignant tumors (leukemia and lymphoma). The immunophenotype and morphology of B-lymphocytes were analyzed directly in the microfluidic chamber, and compared with conventional flow cytometry and visual cytology data, in a blind test. Immunophenotyping results using Ephesia were fully consistent with those obtained by flow cytometry. We obtained in situ high resolution confocal three-dimensional images of the cell nuclei, showing intranuclear details consistent with conventional cytological staining. Ephesia thus provides a powerful approach to cell capture and typing allowing fully automated high resolution and quantitative immunophenotyping and morphological analysis. It requires at least 10 times smaller sample volume and cell numbers than cytometry, potentially increasing the range of indications and the success rate of microbiopsy-based diagnosis, and reducing analysis time and cost.<\/jats:p>","DOI":"10.1073\/pnas.1001515107","type":"journal-article","created":{"date-parts":[[2010,8,2]],"date-time":"2010-08-02T23:35:49Z","timestamp":1280792149000},"page":"14524-14529","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":309,"title":["Microfluidic sorting and multimodal typing of cancer cells in self-assembled magnetic arrays"],"prefix":"10.1073","volume":"107","author":[{"given":"Antoine-Emmanuel","family":"Saliba","sequence":"first","affiliation":[{"name":"Institut Curie, Centre National de la Recherche Scientifique, Universit\u00e9 Pierre et Marie Curie, Unit\u00e9 Mixte de Recherche 168, 75005 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laure","family":"Saias","sequence":"additional","affiliation":[{"name":"Institut Curie, Centre National de la Recherche Scientifique, Universit\u00e9 Pierre et Marie Curie, Unit\u00e9 Mixte de Recherche 168, 75005 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleni","family":"Psychari","sequence":"additional","affiliation":[{"name":"Institut Curie, Centre National de la Recherche Scientifique, Universit\u00e9 Pierre et Marie Curie, Unit\u00e9 Mixte de Recherche 168, 75005 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Minc","sequence":"additional","affiliation":[{"name":"Institut Curie, Centre National de la Recherche Scientifique, Universit\u00e9 Pierre et Marie Curie, Unit\u00e9 Mixte de Recherche 168, 75005 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damien","family":"Simon","sequence":"additional","affiliation":[{"name":"Laboratoire de Physique Statistique, Ecole Normale Sup\u00e9rieure, 75005 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois-Cl\u00e9ment","family":"Bidard","sequence":"additional","affiliation":[{"name":"Institut Curie, D\u00e9partement d'Oncologie M\u00e9dicale, 75005 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Mathiot","sequence":"additional","affiliation":[{"name":"Institut Curie, D\u00e9partement de Biologie des Tumeurs, 75005 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Yves","family":"Pierga","sequence":"additional","affiliation":[{"name":"Institut Curie, D\u00e9partement d'Oncologie M\u00e9dicale, 75005 Paris, France;"},{"name":"Universit\u00e9 Paris Descartes, 75006 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Fraisier","sequence":"additional","affiliation":[{"name":"Institut Curie, Cell and Tissue Imaging platform, Nikon Imaging Centre, Centre National de la Recherche Scientifique, Unit\u00e9 Mixte de Recherche 144, 75005 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Salamero","sequence":"additional","affiliation":[{"name":"Institut Curie, Cell and Tissue Imaging platform, Nikon Imaging Centre, Centre National de la Recherche Scientifique, Unit\u00e9 Mixte de Recherche 144, 75005 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e9ronique","family":"Saada","sequence":"additional","affiliation":[{"name":"Institut de Canc\u00e9rologie Gustave Roussy, D\u00e9partement de Biologie et de Pathologie M\u00e9dicales et Laboratoire de Recherche Translationnelle, 94805 Villejuif, France; 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