{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:37:40Z","timestamp":1781105860388,"version":"3.54.1"},"reference-count":42,"publisher":"National Academy of Sciences","issue":"16","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2010,4,20]]},"abstract":"<jats:p>\n                    Chronic beryllium disease (CBD) is a fibrotic lung disorder caused by beryllium (Be) exposure and is characterized by granulomatous inflammation and the accumulation of Be-responsive CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells in the lung. Genetic susceptibility to CBD has been associated with certain alleles of the MHCII molecule HLA-DP, especially\n                    <jats:italic>HLA-DPB1*0201<\/jats:italic>\n                    and other alleles that contain a glutamic acid residue at position 69 of the \u03b2-chain (\u03b2Glu69). The HLA-DP alleles that can present Be to T cells match those implicated in the genetic susceptibility, suggesting that the HLA contribution to disease is based on the ability of those molecules to bind and present Be to T cells. The structure of HLA-DP2 and its interaction with Be are unknown. Here, we present the HLA-DP2 structure with its antigen-binding groove occupied by a self-peptide derived from the HLA-DR \u03b1-chain. The most striking feature of the structure is an unusual solvent exposed acidic pocket formed between the peptide backbone and the HLA-DP2 \u03b2-chain \u03b1-helix and containing three glutamic acids from the \u03b2-chain, including \u03b2Glu69. In the crystal packing, this pocket has been filled with the guanidinium group of an arginine from a neighboring molecule. This positively charged moiety forms an extensive H-bond\/salt bridge network with the three glutamic acids, offering a plausible model for how Be-containing complexes might occupy this site. This idea is strengthened by the demonstration that mutation of any of the three glutamic acids in this pocket results in loss of the ability of DP2 to present Be to T cells.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1001772107","type":"journal-article","created":{"date-parts":[[2010,3,31]],"date-time":"2010-03-31T23:06:41Z","timestamp":1270076801000},"page":"7425-7430","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":110,"title":["Crystal structure of HLA-DP2 and implications for chronic beryllium disease"],"prefix":"10.1073","volume":"107","author":[{"given":"Shaodong","family":"Dai","sequence":"first","affiliation":[{"name":"Howard Hughes Medical Institute and National Jewish Health, Denver, CO 80206;"},{"name":"Integrated Department of Immunology, National Jewish Health, Denver, CO 80206; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guinevere A.","family":"Murphy","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and National Jewish Health, Denver, CO 80206;"},{"name":"Integrated Department of Immunology, National Jewish Health, Denver, CO 80206; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frances","family":"Crawford","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and National Jewish Health, Denver, CO 80206;"},{"name":"Integrated Department of Immunology, National Jewish Health, Denver, CO 80206; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas G.","family":"Mack","sequence":"additional","affiliation":[{"name":"Departments of cMedicine and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael T.","family":"Falta","sequence":"additional","affiliation":[{"name":"Departments of cMedicine and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippa","family":"Marrack","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and National Jewish Health, Denver, CO 80206;"},{"name":"Integrated Department of Immunology, National Jewish Health, Denver, CO 80206; and"},{"name":"Biochemistry and Molecular Genetics and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John W.","family":"Kappler","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and National Jewish Health, Denver, CO 80206;"},{"name":"Integrated Department of Immunology, National Jewish Health, Denver, CO 80206; and"},{"name":"Program in Structural Biology and Biophysics, University of Colorado, Aurora, CO 80045"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew P.","family":"Fontenot","sequence":"additional","affiliation":[{"name":"Integrated Department of Immunology, National Jewish Health, Denver, CO 80206; and"},{"name":"Departments of cMedicine and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2010,3,31]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1038\/nri1805","article-title":"MHC class II proteins and disease: A structural perspective","volume":"6","author":"Jones EY","year":"2006","unstructured":"EY Jones, L Fugger, JL Strominger, C Siebold, MHC class II proteins and disease: A structural perspective. 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