{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:18:01Z","timestamp":1781108281918,"version":"3.54.1"},"reference-count":50,"publisher":"National Academy of Sciences","issue":"40","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2010,10,5]]},"abstract":"<jats:p>\n                    Phosphatidylinositol-4,5-bisphosphate [PI(4,5)P\n                    <jats:sub>2<\/jats:sub>\n                    ] is a membrane lipid involved in several signaling pathways. However, the role of this lipid in the regulation of synapse growth is ill-defined. Here we identify PI(4,5)P\n                    <jats:sub>2<\/jats:sub>\n                    as a gatekeeper of neuromuscular junction (NMJ) size. We show that PI(4,5)P\n                    <jats:sub>2<\/jats:sub>\n                    levels in neurons are critical in restricting synaptic growth by localizing and activating presynaptic Wiscott-Aldrich syndrome protein\/WASP (WSP). This function of WSP is independent of bone morphogenetic protein (BMP) signaling but is dependent on Tweek, a neuronally expressed protein. Loss of PI(4,5)P\n                    <jats:sub>2<\/jats:sub>\n                    -mediated WSP activation results in increased formation of membrane-organizing extension spike protein (Moesin)-GFP patches that concentrate at sites of bouton growth. Based on pharmacological and genetic studies, Moesin patches mark polymerized actin accumulations and correlate well with NMJ size. We propose a model in which PI(4,5)P\n                    <jats:sub>2<\/jats:sub>\n                    - and WSP-mediated signaling at presynaptic termini controls actin-dependent synapse growth in a pathway at least in part in parallel to synaptic BMP signaling.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1001794107","type":"journal-article","created":{"date-parts":[[2010,9,15]],"date-time":"2010-09-15T22:28:01Z","timestamp":1284589681000},"page":"17379-17384","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":65,"title":["WASP is activated by phosphatidylinositol-4,5-bisphosphate to restrict synapse growth in a pathway parallel to bone morphogenetic protein signaling"],"prefix":"10.1073","volume":"107","author":[{"given":"Thang Manh","family":"Khuong","sequence":"first","affiliation":[{"name":"Department of Molecular and Developmental Genetics, VIB, and"},{"name":"Program in Molecular and Developmental Genetics, and Program in Cognitive and Molecular Neuroscience, Center for Human Genetics, Katholieke Universiteit Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ron L. P.","family":"Habets","sequence":"additional","affiliation":[{"name":"Department of Molecular and Developmental Genetics, VIB, and"},{"name":"Program in Molecular and Developmental Genetics, and Program in Cognitive and Molecular Neuroscience, Center for Human Genetics, Katholieke Universiteit Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan R.","family":"Slabbaert","sequence":"additional","affiliation":[{"name":"Department of Molecular and Developmental Genetics, VIB, and"},{"name":"Program in Molecular and Developmental Genetics, and Program in Cognitive and Molecular Neuroscience, Center for Human Genetics, Katholieke Universiteit Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrik","family":"Verstreken","sequence":"additional","affiliation":[{"name":"Department of Molecular and Developmental Genetics, VIB, and"},{"name":"Program in Molecular and Developmental Genetics, and Program in Cognitive and Molecular Neuroscience, Center for Human Genetics, Katholieke Universiteit Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2010,9,15]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.28.061604.135751"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7742(06)75015-2"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0833-08.2008"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(04)00255-7"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2006.11.018"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)00595-0"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)00589-5"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/neu.10189"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200809127"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(03)00426-4"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn1841"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2008.03.007"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(04)00016-9"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2304-08.2008"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2009.06.017"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.143.2.501"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81560-3"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.06.059"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M000925200"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2006.02.058"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81649-9"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(03)00644-5"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(03)00667-6"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.23.10472"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1223-06.2006"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000277"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.113.21.3685"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2002.0571"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2069"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1006\/clim.1999.4746"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.150.6.1311"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.18.031802.150501"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-01-00002.2003"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11068-008-9024-9"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb856"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.112.1.111"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2008.01.026"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00120-X"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biophys-051309-103849"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.08.030"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1266"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e09-02-0144"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)01014-0"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.7353"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.14.1.231"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2008.169"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.152.1.1-b"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature05954"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200804162"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-178-9_26"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1001794107","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T18:33:32Z","timestamp":1649788412000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1001794107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9,15]]},"references-count":50,"journal-issue":{"issue":"40","published-print":{"date-parts":[[2010,10,5]]}},"alternative-id":["10.1073\/pnas.1001794107"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1001794107","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,9,15]]},"assertion":[{"value":"2010-09-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}