{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T10:59:16Z","timestamp":1790074756546,"version":"4.0.1"},"reference-count":33,"publisher":"National Academy of Sciences","issue":"32","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2010,8,10]]},"abstract":"<jats:p>\n                    Isoprenoids are a large family of compounds with essential functions in all domains of life. Most eubacteria synthesize their isoprenoids using the methylerythritol 4-phosphate (MEP) pathway, whereas a minority uses the unrelated mevalonate pathway and only a few have both. Interestingly,\n                    <jats:italic>Brucella abortus<\/jats:italic>\n                    and some other bacteria that only use the MEP pathway lack deoxyxylulose 5-phosphate (DXP) reductoisomerase (DXR), the enzyme catalyzing the NADPH-dependent production of MEP from DXP in the first committed step of the pathway. Fosmidomycin, a specific competitive inhibitor of DXR, inhibited growth of\n                    <jats:italic>B. abortus<\/jats:italic>\n                    cells expressing the\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    GlpT transporter (required for fosmidomycin uptake), confirming that a DXR-like (DRL) activity exists in these bacteria. The\n                    <jats:italic>B. abortus<\/jats:italic>\n                    DRL protein was found to belong to a family of uncharacterized proteins similar to homoserine dehydrogenase. Subsequent experiments confirmed that DRL and DXR catalyze the same biochemical reaction. DRL homologues shown to complement a DXR-deficient\n                    <jats:italic>E. coli<\/jats:italic>\n                    strain grouped within the same phylogenetic clade. The scattered taxonomic distribution of sequences from the DRL clade and the occurrence of several paralogues in some bacterial strains might be the result of lateral gene transfer and lineage-specific gene duplications and\/or losses, similar to that described for typical mevalonate and MEP pathway genes. These results reveal the existence of a novel class of oxidoreductases catalyzing the conversion of DXP into MEP in prokaryotic cells, underscoring the biochemical and genetic plasticity achieved by bacteria to synthesize essential compounds such as isoprenoids.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1001962107","type":"journal-article","created":{"date-parts":[[2010,7,26]],"date-time":"2010-07-26T23:41:37Z","timestamp":1280187697000},"page":"14081-14086","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":52,"title":["A new family of enzymes catalyzing the first committed step of the methylerythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis in bacteria"],"prefix":"10.1073","volume":"107","author":[{"given":"F\u00e9lix J.","family":"Sangari","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular, Universidad de Cantabria and Instituto de Biomedicina y Biotecnologia de Cantabria (IBBTEC), CSIC-UC-IDICAN, 39011 Santander, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordi","family":"P\u00e9rez-Gil","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB, 08034 Barcelona, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorenzo","family":"Carretero-Paulet","sequence":"additional","affiliation":[{"name":"Department of Applied Biology (Area of Genetics), University of Almer\u00eda, 04120 Almer\u00eda, Spain; and"},{"name":"Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan M.","family":"Garc\u00eda-Lobo","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular, Universidad de Cantabria and Instituto de Biomedicina y Biotecnologia de Cantabria (IBBTEC), CSIC-UC-IDICAN, 39011 Santander, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Rodr\u00edguez-Concepci\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB, 08034 Barcelona, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2010,7,26]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2005.09.003"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5333.1788"},{"key":"e_1_3_3_3_2","first-page":"1250","volume-title":"Biochemistry and Molecular Biology of Plants","author":"Croteau R","year":"2000","unstructured":"R Croteau, T Kutchan, N Lewis, Natural products (secondary metabolites). 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