{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T02:08:45Z","timestamp":1790647725051,"version":"4.1.0"},"reference-count":33,"publisher":"National Academy of Sciences","issue":"25","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2010,6,22]]},"abstract":"<jats:p>\n                    Arthropod-borne viruses (arboviruses) represent a global public health problem, with dengue viruses causing millions of infections annually, while emerging arboviruses, such as West Nile, Japanese encephalitis, and chikungunya viruses have dramatically expanded their geographical ranges. Surveillance of arboviruses provides vital data regarding their prevalence and distribution that may be utilized for biosecurity measures and the implementation of disease control strategies. However, current surveillance methods that involve detection of virus in mosquito populations or sero-conversion in vertebrate hosts are laborious, expensive, and logistically problematic. We report a unique arbovirus surveillance system to detect arboviruses that exploits the process whereby mosquitoes expectorate virus in their saliva during sugar feeding. In this system, infected mosquitoes captured by CO\n                    <jats:sub>2<\/jats:sub>\n                    -baited updraft box traps are allowed to feed on honey-soaked nucleic acid preservation cards within the trap. The cards are then analyzed for expectorated virus using real-time reverse transcription-PCR. In field trials, this system detected the presence of Ross River and Barmah Forest viruses in multiple traps deployed at two locations in Australia. Viral RNA was preserved for at least seven days on the cards, allowing for long-term placement of traps and continuous collection of data documenting virus presence in mosquito populations. Furthermore no mosquito handling or processing was required and cards were conveniently shipped to the laboratory overnight. The simplicity and efficacy of this approach has the potential to transform current approaches to vector-borne disease surveillance by streamlining the monitoring of pathogens in vector populations.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1002040107","type":"journal-article","created":{"date-parts":[[2010,6,7]],"date-time":"2010-06-07T20:17:25Z","timestamp":1275941845000},"page":"11255-11259","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":134,"title":["Exploiting mosquito sugar feeding to detect mosquito-borne pathogens"],"prefix":"10.1073","volume":"107","author":[{"given":"Sonja","family":"Hall-Mendelin","sequence":"first","affiliation":[{"name":"School of Chemistry and Molecular Biosciences, University of Queensland, St. Lucia, Queensland 4072, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott A.","family":"Ritchie","sequence":"additional","affiliation":[{"name":"School of Public Health and Tropical Medicine, James Cook University, Cairns, Queensland 4870, Australia;"},{"name":"Tropical Population Health Unit Network, Queensland Health, Cairns, Queensland 4870, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheryl A.","family":"Johansen","sequence":"additional","affiliation":[{"name":"Discipline of Microbiology and Immunology, School of Biomedical, Biomolecular and Chemical Sciences, University of Western Australia, Nedlands, Western Australia 6009, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Zborowski","sequence":"additional","affiliation":[{"name":"School of Chemistry and Molecular Biosciences, University of Queensland, St. Lucia, Queensland 4072, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giles","family":"Cortis","sequence":"additional","affiliation":[{"name":"Private Contracting Engineer;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott","family":"Dandridge","sequence":"additional","affiliation":[{"name":"The Shire of Harvey, Australind, Western Australia 6233, Australia; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roy A.","family":"Hall","sequence":"additional","affiliation":[{"name":"School of Chemistry and Molecular Biosciences, University of Queensland, St. Lucia, Queensland 4072, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew F.","family":"van den Hurk","sequence":"additional","affiliation":[{"name":"School of Chemistry and Molecular Biosciences, University of Queensland, St. Lucia, Queensland 4072, Australia;"},{"name":"Virology, Queensland Health Forensic and Scientific Services, Coopers Plains, Queensland 4108, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2010,6,7]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)60238-X"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/605496"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1603\/033.046.0601"},{"key":"e_1_3_3_4_2","first-page":"494","article-title":"Persistent West Nile virus transmission and the apparent displacement of St. Louis encephalitis virus in southeastern California, 2003\u20132006","volume":"45","author":"Reisen WK","year":"2008","unstructured":"WK Reisen, et al., Persistent West Nile virus transmission and the apparent displacement of St. Louis encephalitis virus in southeastern California, 2003\u20132006. 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