{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:37:10Z","timestamp":1781109430546,"version":"3.54.1"},"reference-count":52,"publisher":"National Academy of Sciences","issue":"6","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2011,2,8]]},"abstract":"<jats:p>\n                    To clarify the molecular pathways governing hematopoietic stem cell (HSC) development, we screened a fetal liver (FL) HSC cDNA library and identified a unique gene,\n                    <jats:italic>hematopoietic expressed mammalian polycomb<\/jats:italic>\n                    (\n                    <jats:italic>hemp<\/jats:italic>\n                    ), encoding a protein with a zinc-finger domain and four malignant brain tumor (mbt) repeats. To investigate its biological role, we generated mice lacking Hemp (\n                    <jats:italic>hemp<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    ).\n                    <jats:italic>Hemp<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice exhibited a variety of skeletal malformations and died soon after birth. In the FL,\n                    <jats:italic>hemp<\/jats:italic>\n                    was preferentially expressed in the HSC and early progenitor cell fractions, and analyses of fetal hematopoiesis revealed that the number of FL mononuclear cells, including HSCs, was reduced markedly in\n                    <jats:italic>hemp<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    embryos, especially during early development. In addition, colony-forming and competitive repopulation assays demonstrated that the proliferative and reconstitution abilities of\n                    <jats:italic>hemp<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    FL HSCs were significantly impaired. Microarray analysis revealed alterations in the expression levels of several genes implicated in hematopoietic development and differentiation in\n                    <jats:italic>hemp<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    FL HSCs. These results demonstrate that Hemp, an mbt-containing protein, plays essential roles in HSC function and skeletal formation. It is also hypothesized that Hemp might be involved in certain congenital diseases, such as Klippel-Feil anomaly.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1003403108","type":"journal-article","created":{"date-parts":[[2011,1,20]],"date-time":"2011-01-20T23:01:02Z","timestamp":1295564462000},"page":"2468-2473","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":19,"title":["Hemp, an mbt domain-containing protein, plays essential roles in hematopoietic stem cell function and skeletal formation"],"prefix":"10.1073","volume":"108","author":[{"given":"Hiroaki","family":"Honda","sequence":"first","affiliation":[{"name":"Department of Disease Model, Research Institute of Radiation Biology and Medicine, Hiroshima University, Minami-ku, Hiroshima 734-8553, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiyo","family":"Takubo","sequence":"additional","affiliation":[{"name":"Department of Cell 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Research Institute of Radiation Biology and Medicine, Hiroshima University, Minami-ku, Hiroshima 734-8553, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuko","family":"Miyazaki","sequence":"additional","affiliation":[{"name":"Department of Disease Model, Research Institute of Radiation Biology and Medicine, Hiroshima University, Minami-ku, Hiroshima 734-8553, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kateri A.","family":"Moore","sequence":"additional","affiliation":[{"name":"Department of Gene and Cell Medicine, Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York, NY 10029; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zen-ichiro","family":"Honda","sequence":"additional","affiliation":[{"name":"Department of Allergy and Rheumatology, Faculty of Medicine, Graduate School of Medicine, University of Tokyo, Bunkyo-ku, Tokyo 113-8655, 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