{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T07:13:37Z","timestamp":1789629217341,"version":"build-2803163510"},"reference-count":51,"publisher":"National Academy of Sciences","issue":"30","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2010,7,27]]},"abstract":"<jats:p>Thymus organogenesis requires coordinated interactions of multiple cell types, including neural crest (NC) cells, to orchestrate the formation, separation, and subsequent migration of the developing thymus from the third pharyngeal pouch to the thoracic cavity. The molecular mechanisms driving these processes are unclear; however, NC-derived mesenchyme has been shown to play an important role. Here, we show that, in the absence of ephrin-B2 expression on thymic NC-derived mesenchyme, the thymus remains in the cervical area instead of migrating into the thoracic cavity. Analysis of individual NC-derived thymic mesenchymal cells shows that, in the absence of ephrin-B2, their motility is impaired as a result of defective EphB receptor signaling. This implies a NC-derived cell-specific role of EphB\u2013ephrin-B2 interactions in the collective migration of the thymic rudiment during organogenesis.<\/jats:p>","DOI":"10.1073\/pnas.1003747107","type":"journal-article","created":{"date-parts":[[2010,7,8]],"date-time":"2010-07-08T22:58:34Z","timestamp":1278629914000},"page":"13414-13419","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":51,"title":["EphB\u2013ephrin-B2 interactions are required for thymus migration during organogenesis"],"prefix":"10.1073","volume":"107","author":[{"given":"Katie E.","family":"Foster","sequence":"first","affiliation":[{"name":"Molecular Immunology, National Institute of Medical Research, London NW7 1AA, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Gordon","sequence":"additional","affiliation":[{"name":"Department of Genetics, University of Georgia, Athens, GA 30602;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim","family":"Cardenas","sequence":"additional","affiliation":[{"name":"Science Park\u2013Research Division, University of Texas MD Anderson Cancer Center, Smithville, TX 78957;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrique","family":"Veiga-Fernandes","sequence":"additional","affiliation":[{"name":"Molecular Immunology, National Institute of Medical Research, London NW7 1AA, United Kingdom;"},{"name":"Faculdade de Medicina de Lisboa, Instituto de Medicina Molecular, 1649-020 Lisbon, Portugal;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taija","family":"Makinen","sequence":"additional","affiliation":[{"name":"Lymphatic Development Laboratory, Cancer Research UK\u2013London Research Institute, London WC2A 3PX, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Grigorieva","sequence":"additional","affiliation":[{"name":"Developmental Neurobiology, National Institute of Medical Research, London NW7 1AA, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David G.","family":"Wilkinson","sequence":"additional","affiliation":[{"name":"Developmental Neurobiology, National Institute of Medical Research, London NW7 1AA, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Clare","family":"Blackburn","sequence":"additional","affiliation":[{"name":"Medical Research Council Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JQ, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen","family":"Richie","sequence":"additional","affiliation":[{"name":"Science Park\u2013Research Division, University of Texas MD Anderson Cancer Center, Smithville, TX 78957;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy R.","family":"Manley","sequence":"additional","affiliation":[{"name":"Department of Genetics, University of Georgia, Athens, GA 30602;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf H.","family":"Adams","sequence":"additional","affiliation":[{"name":"Max-Planck Institute for Molecular Biomedicine, Department of Tissue Morphogenesis, University of Munster, Munster, Germany, D-48149; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitris","family":"Kioussis","sequence":"additional","affiliation":[{"name":"Molecular Immunology, National Institute of Medical Research, London NW7 1AA, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark C.","family":"Coles","sequence":"additional","affiliation":[{"name":"Molecular Immunology, National Institute of Medical Research, London NW7 1AA, United Kingdom;"},{"name":"Centre for Immunology and Infection, Department of Biology and Hull York Medical School, University of York, York YO10 5YW, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2010,7,8]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122.1.23"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2001.0342"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.17.2735"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2005.12.015"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.12.019"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.4.1954"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.5.3183"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.8.5344"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200204)32:4<1175::AID-IMMU1175>3.0.CO;2-U"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1997.8827"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.9.1869"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-6-51"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.3.295"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81436-1"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.10.2033"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(00)96005-4"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00191-X"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1171704"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00625"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2003.10.006"},{"key":"e_1_3_3_21_2","first-page":"897","article-title":"cDNA cloning and tissue distribution of five human EPH-like receptor protein-tyrosine kinases","volume":"10","author":"Fox GM","year":"1995","unstructured":"GM Fox, et al., cDNA cloning and tissue distribution of five human EPH-like receptor protein-tyrosine kinases. Oncogene 10, 897\u2013905 (1995).","journal-title":"Oncogene"},{"key":"e_1_3_3_22_2","first-page":"777","article-title":"A new member of the Eph family of receptors that lacks protein tyrosine kinase activity","volume":"13","author":"Gurniak CB","year":"1996","unstructured":"CB Gurniak, LJ Berg, A new member of the Eph family of receptors that lacks protein tyrosine kinase activity. Oncogene 13, 777\u2013786 (1996).","journal-title":"Oncogene"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI15883"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-133X(02)00054-6"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.1.106"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.moh.0000166497.26397.9f"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.2.804"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M208179200"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2008.02828.x"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200737097"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn1164"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20497"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(07)00562-3"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-1-4"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.12.028"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.1.177"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.11.6484"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1123497"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0985-04.2004"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M701319200"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00314-2"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn964"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)01168-6"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402610107"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(03)00036-9"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02738240"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092270414"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)00683-9"},{"key":"e_1_3_3_49_2","first-page":"2125","article-title":"Collective cell movement in primary melanoma explants: Plasticity of cell\u2013cell interaction, beta1-integrin function, and migration strategies","volume":"62","author":"Hegerfeldt Y","year":"2002","unstructured":"Y Hegerfeldt, M Tusch, EB Br\u00f6cker, P Friedl, Collective cell movement in primary melanoma explants: Plasticity of cell\u2013cell interaction, beta1-integrin function, and migration strategies. Cancer Res 62, 2125\u20132130 (2002).","journal-title":"Cancer Res"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-7-69"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.330105"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1003747107","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T18:59:55Z","timestamp":1649789995000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1003747107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,7,8]]},"references-count":51,"journal-issue":{"issue":"30","published-print":{"date-parts":[[2010,7,27]]}},"alternative-id":["10.1073\/pnas.1003747107"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1003747107","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,7,8]]},"assertion":[{"value":"2010-07-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}