{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T04:15:37Z","timestamp":1792728937217,"version":"4.5.0"},"reference-count":26,"publisher":"National Academy of Sciences","issue":"44","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2010,11,2]]},"abstract":"<jats:p>\n                    <jats:italic>Coxiella burnetii<\/jats:italic>\n                    and\n                    <jats:italic>Legionella pneumophila<\/jats:italic>\n                    are evolutionarily related pathogens with different intracellular infection strategies.\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    persists within and is transmitted by mammalian hosts, whereas,\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    is found primarily in the environment associated with protozoan hosts. Although a type IV secretion system encoded by the defect in organelle trafficking (\n                    <jats:italic>dot<\/jats:italic>\n                    ) and intracellular multiplication (\n                    <jats:italic>icm<\/jats:italic>\n                    ) genes is a virulence determinant that remains highly conserved in both bacteria, the two pathogens encode a different array of effector proteins that are delivered into host cells by the Dot\/Icm machinery. This difference suggests that adaptations to evolutionarily distinct hosts may be reflected in the effector protein repertoires displayed by these two pathogens. Here we provide evidence in support of this hypothesis. We show that a unique\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    effector from the ankyrin repeat (Ank) family called AnkG interferes with the mammalian apoptosis pathway. AnkG was found to interact with the host protein gC1qR (p32). Either the addition of AnkG to the repertoire of\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    effector proteins or the silencing of p32 in mouse dendritic cells resulted in a gain of function that allowed intracellular replication of\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    in these normally restrictive mammalian host cells by preventing rapid pathogen-induced apoptosis. These data indicate that p32 regulates pathogen-induced apoptosis and that AnkG functions to block this pathway. Thus, emergence of an effector protein that interferes with a proapoptotic signaling pathway directed against intracellular bacteria correlates with adaptation of a pathogen to mammalian hosts.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1004380107","type":"journal-article","created":{"date-parts":[[2010,10,14]],"date-time":"2010-10-14T03:28:13Z","timestamp":1287026893000},"page":"18997-19001","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":166,"title":["Inhibition of pathogen-induced apoptosis by a\n                    <i>Coxiella burnetii<\/i>\n                    type IV effector protein"],"prefix":"10.1073","volume":"107","author":[{"given":"Anja","family":"L\u00fchrmann","sequence":"first","affiliation":[{"name":"Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06536;"},{"name":"Microbiology Institute, University Clinic Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 91054 Erlangen, Germany; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catarina V.","family":"Nogueira","sequence":"additional","affiliation":[{"name":"Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06536;"},{"name":"Graduate Program in Areas of Basic and Applied Biology, Instituto de Ci\u00eancias Biomedicas Dr. Abel Salazar, Universidade do Porto, 4099-003 Porto, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly L.","family":"Carey","sequence":"additional","affiliation":[{"name":"Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06536;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig R.","family":"Roy","sequence":"additional","affiliation":[{"name":"Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06536;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2010,10,13]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/0966-842X(96)10041-X"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2007.06.006"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2008.12.004"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1967"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197712012972201"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2008.01145.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000478"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1646"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20051659"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1305"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0020018"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.8.4202-4206.1989"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0931379100"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1099776"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1447"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01141-08"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00863-07"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00594-07"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1158160"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01656-08"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4401418"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-006-5396-4"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)00136-2"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.7.3572"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2008.04.002"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2008.04.004"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1004380107","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T18:53:43Z","timestamp":1649789623000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1004380107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,10,13]]},"references-count":26,"journal-issue":{"issue":"44","published-print":{"date-parts":[[2010,11,2]]}},"alternative-id":["10.1073\/pnas.1004380107"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1004380107","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,10,13]]},"assertion":[{"value":"2010-10-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}