{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T23:07:50Z","timestamp":1792019270810,"version":"4.4.0"},"reference-count":41,"publisher":"National Academy of Sciences","issue":"29","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2010,7,20]]},"abstract":"<jats:p>\n                    The multisubunit Golgi-associated retrograde protein (GARP) complex is required for tethering and fusion of endosome-derived transport vesicles to the\n                    <jats:italic>trans<\/jats:italic>\n                    -Golgi network. Mutation of leucine-967 to glutamine in the Vps54 subunit of GARP is responsible for spinal muscular atrophy in the wobbler mouse, an animal model of amyotrophic lateral sclerosis. The crystal structure at 1.7\u00a0\u212b resolution of the mouse Vps54 C-terminal fragment harboring leucine-967, in conjunction with comparative sequence analysis, reveals that Vps54 has a continuous \u03b1-helical bundle organization similar to that of other multisubunit tethering complexes. The structure shows that leucine-967 is buried within the \u03b1-helical bundle through predominantly hydrophobic interactions that are critical for domain stability and folding in vitro. Mutation of this residue to glutamine does not prevent integration of Vps54 into the GARP complex but greatly reduces the half-life and levels of the protein in vivo. Severely reduced levels of mutant Vps54 and, consequently, of the whole GARP complex underlie the phenotype of the wobbler mouse.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1004756107","type":"journal-article","created":{"date-parts":[[2010,7,6]],"date-time":"2010-07-06T22:23:58Z","timestamp":1278455038000},"page":"12860-12865","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":74,"title":["Structural basis for the wobbler mouse neurodegenerative disorder caused by mutation in the Vps54 subunit of the GARP complex"],"prefix":"10.1073","volume":"107","author":[{"given":"F. Javier","family":"P\u00e9rez-Victoria","sequence":"first","affiliation":[{"name":"Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermo","family":"Abascal-Palacios","sequence":"additional","affiliation":[{"name":"Structural Biology Unit, Center for Cooperative Research in Biosciences bioGUNE, Bizkaia Technology Park, 48160 Derio, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor","family":"Tasc\u00f3n","sequence":"additional","affiliation":[{"name":"Structural Biology Unit, Center for Cooperative Research in Biosciences bioGUNE, Bizkaia Technology Park, 48160 Derio, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey","family":"Kajava","sequence":"additional","affiliation":[{"name":"Centre de Recherches de Biochimie Macromol\u00e9culaire, Centre National de la Recherche Scientifique, University of Montpellier, 34293 Montpellier, France; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier G.","family":"Magad\u00e1n","sequence":"additional","affiliation":[{"name":"Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik P.","family":"Pioro","sequence":"additional","affiliation":[{"name":"Neuromuscular Center, Neurological Institute, Cleveland Clinic, Cleveland, OH 44195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan S.","family":"Bonifacino","sequence":"additional","affiliation":[{"name":"Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aitor","family":"Hierro","sequence":"additional","affiliation":[{"name":"Structural Biology Unit, Center for Cooperative Research in Biosciences bioGUNE, Bizkaia Technology Park, 48160 Derio, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2010,7,6]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1016\/j.devcel.2007.04.005","article-title":"Coats, tethers, Rabs, and SNAREs work together to mediate the intracellular destination of a transport vesicle","volume":"12","author":"Cai H","year":"2007","unstructured":"H Cai, K Reinisch, S Ferro-Novick, Coats, tethers, Rabs, and SNAREs work together to mediate the intracellular destination of a transport vesicle. Dev Cell 12, 671\u2013682 (2007).","journal-title":"Dev Cell"},{"key":"e_1_3_4_2_2","doi-asserted-by":"crossref","first-page":"E17","DOI":"10.1038\/8967","article-title":"Transport-vesicle targeting: Tethers before SNAREs","volume":"1","author":"Pfeffer SR","year":"1999","unstructured":"SR Pfeffer, Transport-vesicle targeting: Tethers before SNAREs. Nat Cell Biol 1, E17\u201322 (1999).","journal-title":"Nat Cell Biol"},{"key":"e_1_3_4_3_2","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.bbamcr.2005.03.013","article-title":"Golgi tethering factors","volume":"1744","author":"Lupashin V","year":"2005","unstructured":"V Lupashin, E Sztul, Golgi tethering factors. Biochim Biophys Acta 1744, 325\u2013339 (2005).","journal-title":"Biochim Biophys Acta"},{"key":"e_1_3_4_4_2","doi-asserted-by":"crossref","first-page":"C11","DOI":"10.1152\/ajpcell.00293.2005","article-title":"Role of tethering factors in secretory membrane traffic","volume":"290","author":"Sztul E","year":"2006","unstructured":"E Sztul, V Lupashin, Role of tethering factors in secretory membrane traffic. Am J Physiol\u2014Cell Physiol 290, C11\u201326 (2006).","journal-title":"Am J Physiol\u2014Cell Physiol"},{"key":"e_1_3_4_5_2","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1038\/nsmb1097","article-title":"The exocyst defrocked, a framework of rods revealed","volume":"13","author":"Munson M","year":"2006","unstructured":"M Munson, P Novick, The exocyst defrocked, a framework of rods revealed. Nat Struct Mol Biol 13, 577\u2013581 (2006).","journal-title":"Nat Struct Mol Biol"},{"key":"e_1_3_4_6_2","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/j.ceb.2009.05.007","article-title":"Vps-C complexes: Gatekeepers of endolysosomal traffic","volume":"21","author":"Nickerson DP","year":"2009","unstructured":"DP Nickerson, CL Brett, AJ Merz, Vps-C complexes: Gatekeepers of endolysosomal traffic. Curr Opin Cell Biol 21, 543\u2013551 (2009).","journal-title":"Curr Opin Cell Biol"},{"key":"e_1_3_4_7_2","doi-asserted-by":"crossref","first-page":"3770","DOI":"10.1016\/j.febslet.2009.10.083","article-title":"Role of vesicle tethering factors in the ER-Golgi membrane traffic","volume":"583","author":"Sztul E","year":"2009","unstructured":"E Sztul, V Lupashin, Role of vesicle tethering factors in the ER-Golgi membrane traffic. FEBS Lett 583, 3770\u20133783 (2009).","journal-title":"FEBS Lett"},{"key":"e_1_3_4_8_2","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/S1534-5807(01)00063-6","article-title":"The Sec34\/35 Golgi transport complex is related to the exocyst, defining a family of complexes involved in multiple steps of membrane traffic","volume":"1","author":"Whyte JR","year":"2001","unstructured":"JR Whyte, S Munro, The Sec34\/35 Golgi transport complex is related to the exocyst, defining a family of complexes involved in multiple steps of membrane traffic. Dev Cell 1, 527\u2013537 (2001).","journal-title":"Dev Cell"},{"key":"e_1_3_4_9_2","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1186\/1471-2148-7-29","article-title":"Control systems for membrane fusion in the ancestral eukaryote; evolution of tethering complexes and SM proteins","volume":"7","author":"Koumandou VL","year":"2007","unstructured":"VL Koumandou, JB Dacks, RM Coulson, MC Field, Control systems for membrane fusion in the ancestral eukaryote; evolution of tethering complexes and SM proteins. BMC Evol Biol 7, 29 (2007).","journal-title":"BMC Evol Biol"},{"key":"e_1_3_4_10_2","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/j.jmb.2009.06.054","article-title":"Remote homology between Munc13 MUN domain and vesicle tethering complexes","volume":"391","author":"Pei J","year":"2009","unstructured":"J Pei, C Ma, J Rizo, NV Grishin, Remote homology between Munc13 MUN domain and vesicle tethering complexes. J Mol Biol 391, 509\u2013517 (2009).","journal-title":"J Mol Biol"},{"key":"e_1_3_4_11_2","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1038\/nsmb1017","article-title":"The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif","volume":"12","author":"Dong G","year":"2005","unstructured":"G Dong, AH Hutagalung, C Fu, P Novick, KM Reinisch, The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif. Nat Struct Mol Biol 12, 1094\u20131100 (2005).","journal-title":"Nat Struct Mol Biol"},{"key":"e_1_3_4_12_2","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1016\/j.cell.2009.11.002","article-title":"A structure-based mechanism for vesicle capture by the multisubunit tethering complex Dsl1","volume":"139","author":"Ren Y","year":"2009","unstructured":"Y Ren, et al., A structure-based mechanism for vesicle capture by the multisubunit tethering complex Dsl1. Cell 139, 1119\u20131129 (2009).","journal-title":"Cell"},{"key":"e_1_3_4_13_2","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1038\/nsmb.1548","article-title":"Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex","volume":"16","author":"Tripathi A","year":"2009","unstructured":"A Tripathi, Y Ren, PD Jeffrey, FM Hughson, Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nat Struct Mol Biol 16, 114\u2013123 (2009).","journal-title":"Nat Struct Mol Biol"},{"key":"e_1_3_4_14_2","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1091\/mbc.11.1.305","article-title":"Vps52p, Vps53p, and Vps54p form a novel multisubunit complex required for protein sorting at the yeast late Golgi","volume":"11","author":"Conibear E","year":"2000","unstructured":"E Conibear, TH Stevens, Vps52p, Vps53p, and Vps54p form a novel multisubunit complex required for protein sorting at the yeast late Golgi. Mol Biol Cell 11, 305\u2013323 (2000).","journal-title":"Mol Biol Cell"},{"key":"e_1_3_4_15_2","doi-asserted-by":"crossref","first-page":"48318","DOI":"10.1074\/jbc.M209428200","article-title":"Vps51p links the VFT complex to the SNARE Tlg1p","volume":"277","author":"Siniossoglou S","year":"2002","unstructured":"S Siniossoglou, HR Pelham, Vps51p links the VFT complex to the SNARE Tlg1p. J Biol Chem 277, 48318\u201348324 (2002).","journal-title":"J Biol Chem"},{"key":"e_1_3_4_16_2","doi-asserted-by":"crossref","first-page":"1610","DOI":"10.1091\/mbc.e02-10-0654","article-title":"Vps51p mediates the association of the GARP (Vps52\/53\/54) complex with the late Golgi t-SNARE Tlg1p","volume":"14","author":"Conibear E","year":"2003","unstructured":"E Conibear, JN Cleck, TH Stevens, Vps51p mediates the association of the GARP (Vps52\/53\/54) complex with the late Golgi t-SNARE Tlg1p. Mol Biol Cell 14, 1610\u20131623 (2003).","journal-title":"Mol Biol Cell"},{"key":"e_1_3_4_17_2","doi-asserted-by":"crossref","first-page":"5009","DOI":"10.1074\/jbc.M210436200","article-title":"Vps51 is part of the yeast Vps fifty-three tethering complex essential for retrograde traffic from the early endosome and Cvt vesicle completion","volume":"278","author":"Reggiori F","year":"2003","unstructured":"F Reggiori, CW Wang, PE Stromhaug, T Shintani, DJ Klionsky, Vps51 is part of the yeast Vps fifty-three tethering complex essential for retrograde traffic from the early endosome and Cvt vesicle completion. J Biol Chem 278, 5009\u20135020 (2003).","journal-title":"J Biol Chem"},{"key":"e_1_3_4_18_2","doi-asserted-by":"crossref","first-page":"2350","DOI":"10.1091\/mbc.e07-11-1189","article-title":"Requirement of the human GARP complex for mannose 6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes","volume":"19","author":"Perez-Victoria FJ","year":"2008","unstructured":"FJ Perez-Victoria, GA Mardones, JS Bonifacino, Requirement of the human GARP complex for mannose 6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes. Mol Biol Cell 19, 2350\u20132362 (2008).","journal-title":"Mol Biol Cell"},{"key":"e_1_3_4_19_2","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1038\/ng1661","article-title":"Mutation of Vps54 causes motor neuron disease and defective spermiogenesis in the wobbler mouse","volume":"37","author":"Schmitt-John T","year":"2005","unstructured":"T Schmitt-John, et al., Mutation of Vps54 causes motor neuron disease and defective spermiogenesis in the wobbler mouse. Nat Genet 37, 1213\u20131215 (2005).","journal-title":"Nat Genet"},{"key":"e_1_3_4_20_2","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1385\/MN:28:1:65","article-title":"The wobbler mouse: A neurodegeneration jigsaw puzzle","volume":"28","author":"Boillee S","year":"2003","unstructured":"S Boillee, M Peschanski, MP Junier, The wobbler mouse: A neurodegeneration jigsaw puzzle. Mol Neurobiol 28, 65\u2013106 (2003).","journal-title":"Mol Neurobiol"},{"key":"e_1_3_4_21_2","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1080\/17482960801934403","article-title":"Evaluation of the Golgi trafficking protein VPS54 (wobbler) as a candidate for ALS","volume":"9","author":"Meisler MH","year":"2008","unstructured":"MH Meisler, et al., Evaluation of the Golgi trafficking protein VPS54 (wobbler) as a candidate for ALS. Amyotroph Lateral Sc 9, 141\u2013148 (2008).","journal-title":"Amyotroph Lateral Sc"},{"key":"e_1_3_4_22_2","doi-asserted-by":"crossref","first-page":"2780","DOI":"10.1093\/bioinformatics\/btn507","article-title":"Searching protein structure databases with DaliLite v.3.","volume":"24","author":"Holm L","year":"2008","unstructured":"L Holm, S Kaariainen, P Rosenstrom, A Schenkel, Searching protein structure databases with DaliLite v.3. Bioinformatics 24, 2780\u20132781 (2008).","journal-title":"Bioinformatics"},{"key":"e_1_3_4_23_2","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1038\/nsmb1096","article-title":"The structure of the exocyst subunit Sec6p defines a conserved architecture with diverse roles","volume":"13","author":"Sivaram MV","year":"2006","unstructured":"MV Sivaram, ML Furgason, DN Brewer, M Munson, The structure of the exocyst subunit Sec6p defines a conserved architecture with diverse roles. Nat Struct Mol Biol 13, 555\u2013556 (2006).","journal-title":"Nat Struct Mol Biol"},{"key":"e_1_3_4_24_2","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1038\/nsmb987","article-title":"Sec15 interacts with Rab11 via a novel domain and affects Rab11 localization in vivo","volume":"12","author":"Wu S","year":"2005","unstructured":"S Wu, SQ Mehta, F Pichaud, HJ Bellen, FA Quiocho, Sec15 interacts with Rab11 via a novel domain and affects Rab11 localization in vivo. Nat Struct Mol Biol 12, 879\u2013885 (2005).","journal-title":"Nat Struct Mol Biol"},{"key":"e_1_3_4_25_2","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1016\/j.jmb.2007.05.018","article-title":"The crystal structure of mouse Exo70 reveals unique features of the mammalian exocyst","volume":"371","author":"Moore BA","year":"2007","unstructured":"BA Moore, HH Robinson, Z Xu, The crystal structure of mouse Exo70 reveals unique features of the mammalian exocyst. J Mol Biol 371, 410\u2013421 (2007).","journal-title":"J Mol Biol"},{"key":"e_1_3_4_26_2","doi-asserted-by":"crossref","first-page":"23418","DOI":"10.1074\/jbc.M703716200","article-title":"Structural analysis of conserved oligomeric Golgi complex subunit 2","volume":"282","author":"Cavanaugh LF","year":"2007","unstructured":"LF Cavanaugh, et al., Structural analysis of conserved oligomeric Golgi complex subunit 2. J Biol Chem 282, 23418\u201323426 (2007).","journal-title":"J Biol Chem"},{"key":"e_1_3_4_27_2","doi-asserted-by":"crossref","first-page":"13329","DOI":"10.1073\/pnas.0901966106","article-title":"Structural basis for a human glycosylation disorder caused by mutation of the COG4 gene","volume":"106","author":"Richardson BC","year":"2009","unstructured":"BC Richardson, et al., Structural basis for a human glycosylation disorder caused by mutation of the COG4 gene. Proc Natl Acad Sci USA 106, 13329\u201313334 (2009).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_28_2","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1006\/jmbi.1998.2342","article-title":"A correlation between the loss of hydrophobic core packing interactions and protein stability","volume":"285","author":"Vlassi M","year":"1999","unstructured":"M Vlassi, G Cesareni, M Kokkinidis, A correlation between the loss of hydrophobic core packing interactions and protein stability. J Mol Biol 285, 817\u2013827 (1999).","journal-title":"J Mol Biol"},{"key":"e_1_3_4_29_2","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1002\/bip.21316","article-title":"Intrinsic amino acid side-chain hydrophilicity\/hydrophobicity coefficients determined by reversed-phase high-performance liquid chromatography of model peptides: Comparison with other hydrophilicity\/hydrophobicity scales","volume":"92","author":"Mant CT","year":"2009","unstructured":"CT Mant, JM Kovacs, HM Kim, DD Pollock, RS Hodges, Intrinsic amino acid side-chain hydrophilicity\/hydrophobicity coefficients determined by reversed-phase high-performance liquid chromatography of model peptides: Comparison with other hydrophilicity\/hydrophobicity scales. Biopolymers 92, 573\u2013595 (2009).","journal-title":"Biopolymers"},{"key":"e_1_3_4_30_2","doi-asserted-by":"crossref","first-page":"1859","DOI":"10.1091\/mbc.e05-11-1002","article-title":"Domains within the GARP subunit Vps54 confer separate functions in complex assembly and early endosome recognition","volume":"17","author":"Quenneville NR","year":"2006","unstructured":"NR Quenneville, TY Chao, JM McCaffery, E Conibear, Domains within the GARP subunit Vps54 confer separate functions in complex assembly and early endosome recognition. Mol Biol Cell 17, 1859\u20131870 (2006).","journal-title":"Mol Biol Cell"},{"key":"e_1_3_4_31_2","doi-asserted-by":"crossref","first-page":"5251","DOI":"10.1128\/MCB.00495-09","article-title":"Dual roles of the mammalian GARP complex in tethering and SNARE complex assembly at the trans-Golgi network","volume":"29","author":"Perez-Victoria FJ","year":"2009","unstructured":"FJ Perez-Victoria, JS Bonifacino, Dual roles of the mammalian GARP complex in tethering and SNARE complex assembly at the trans-Golgi network. Mol Cell Biol 29, 5251\u20135263 (2009).","journal-title":"Mol Cell Biol"},{"key":"e_1_3_4_32_2","doi-asserted-by":"crossref","first-page":"113","DOI":"10.4161\/auto.5227","article-title":"Lysosomal storage diseases as disorders of autophagy","volume":"4","author":"Settembre C","year":"2008","unstructured":"C Settembre, A Fraldi, DC Rubinsztein, A Ballabio, Lysosomal storage diseases as disorders of autophagy. Autophagy 4, 113\u2013114 (2008).","journal-title":"Autophagy"},{"key":"e_1_3_4_33_2","doi-asserted-by":"crossref","first-page":"6898","DOI":"10.1523\/JNEUROSCI.20-18-06898.2000","article-title":"Cathepsin D deficiency induces lysosomal storage with ceroid lipofuscin in mouse CNS neurons","volume":"20","author":"Koike M","year":"2000","unstructured":"M Koike, et al., Cathepsin D deficiency induces lysosomal storage with ceroid lipofuscin in mouse CNS neurons. J Neurosci 20, 6898\u20136906 (2000).","journal-title":"J Neurosci"},{"key":"e_1_3_4_34_2","doi-asserted-by":"crossref","first-page":"2786","DOI":"10.1093\/emboj\/19.12.2786","article-title":"A mutation in the ovine cathepsin D gene causes a congenital lysosomal storage disease with profound neurodegeneration","volume":"19","author":"Tyynela J","year":"2000","unstructured":"J Tyynela, et al., A mutation in the ovine cathepsin D gene causes a congenital lysosomal storage disease with profound neurodegeneration. EMBO J 19, 2786\u20132792 (2000).","journal-title":"EMBO J"},{"key":"e_1_3_4_35_2","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1006\/exnr.1996.6364","article-title":"TRPM-2 expression and tunel staining in neurodegenerative diseases: Studies in wobbler and rd mice","volume":"143","author":"Popper P","year":"1997","unstructured":"P Popper, DB Farber, PE Micevych, K Minoofar, JM Bronstein, TRPM-2 expression and tunel staining in neurodegenerative diseases: Studies in wobbler and rd mice. Exp Neurol 143, 246\u2013254 (1997).","journal-title":"Exp Neurol"},{"key":"e_1_3_4_36_2","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1006\/nbdi.2002.0564","article-title":"Progesterone neuroprotection in the Wobbler mouse, a genetic model of spinal cord motor neuron disease","volume":"11","author":"Gonzalez Deniselle MC","year":"2002","unstructured":"MC Gonzalez Deniselle, et al., Progesterone neuroprotection in the Wobbler mouse, a genetic model of spinal cord motor neuron disease. Neurobiol Dis 11, 457\u2013468 (2002).","journal-title":"Neurobiol Dis"},{"key":"e_1_3_4_37_2","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.neulet.2007.08.057","article-title":"Lack of caspase-dependent apoptosis in spinal motor neurons of the wobbler mouse","volume":"426","author":"Bigini P","year":"2007","unstructured":"P Bigini, et al., Lack of caspase-dependent apoptosis in spinal motor neurons of the wobbler mouse. Neurosci Lett 426, 106\u2013110 (2007).","journal-title":"Neurosci Lett"},{"key":"e_1_3_4_38_2","doi-asserted-by":"crossref","first-page":"2052","DOI":"10.1073\/pnas.0708022105","article-title":"Lithium delays progression of amyotrophic lateral sclerosis","volume":"105","author":"Fornai F","year":"2008","unstructured":"F Fornai, et al., Lithium delays progression of amyotrophic lateral sclerosis. Proc Natl Acad Sci USA 105, 2052\u20132057 (2008).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_39_2","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1107\/S0907444994003112","article-title":"The CCP4 suite: Programs for protein crystallography","volume":"50","year":"1994","unstructured":", The CCP4 suite: Programs for protein crystallography. Acta Crystallogr D 50, 760\u2013763 (1994).","journal-title":"Acta Crystallogr D"},{"key":"e_1_3_4_40_2","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.1107\/S0907444904019158","article-title":"Coot: Model-building tools for molecular graphics","volume":"60","author":"Emsley P","year":"2004","unstructured":"P Emsley, K Cowtan, Coot: Model-building tools for molecular graphics. Acta Crystallogr D 60, 2126\u20132132 (2004).","journal-title":"Acta Crystallogr D"},{"key":"e_1_3_4_41_2","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","article-title":"PHENIX: A comprehensive Python-based system for macromolecular structure solution","volume":"66","author":"Adams PD","unstructured":"PD Adams, et al., PHENIX: A comprehensive Python-based system for macromolecular structure solution. Acta Crystallogr D 66, 213\u2013221","journal-title":"Acta Crystallogr D"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1004756107","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T02:55:26Z","timestamp":1654570526000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1004756107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,7,6]]},"references-count":41,"journal-issue":{"issue":"29","published-print":{"date-parts":[[2010,7,20]]}},"alternative-id":["10.1073\/pnas.1004756107"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1004756107","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,7,6]]},"assertion":[{"value":"2010-07-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}