{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T00:22:59Z","timestamp":1793319779718,"version":"4.5.0"},"reference-count":24,"publisher":"National Academy of Sciences","issue":"42","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2010,10,19]]},"abstract":"<jats:p>\n                    Salicylic acid (SA) is a defense hormone required for both local and systemic acquired resistance (SAR) in plants. Pathogen infections induce SA synthesis through up-regulating the expression of\n                    <jats:italic>Isochorismate Synthase 1<\/jats:italic>\n                    (\n                    <jats:italic>ICS1<\/jats:italic>\n                    ), which encodes a key enzyme in SA production. Here we report that both SAR Deficient 1 (SARD1) and CBP60g are key regulators for\n                    <jats:italic>ICS1<\/jats:italic>\n                    induction and SA synthesis. Whereas knocking out\n                    <jats:italic>SARD1<\/jats:italic>\n                    compromises basal resistance and SAR, overexpression of\n                    <jats:italic>SARD1<\/jats:italic>\n                    constitutively activates defense responses. In the\n                    <jats:italic>sard1-1 cbp60g-1<\/jats:italic>\n                    double mutant, pathogen-induced\n                    <jats:italic>ICS1<\/jats:italic>\n                    up-regulation and SA synthesis are blocked in both local and systemic leaves, resulting in compromised basal resistance and loss of SAR. Electrophoretic mobility shift assays showed that SARD1 and CBP60g represent a plant-specific family of DNA-binding proteins. Both proteins are recruited to the promoter of\n                    <jats:italic>ICS1<\/jats:italic>\n                    in response to pathogen infections, suggesting that they control SA synthesis by regulating\n                    <jats:italic>ICS1<\/jats:italic>\n                    at the transcriptional level.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1005225107","type":"journal-article","created":{"date-parts":[[2010,10,4]],"date-time":"2010-10-04T22:41:48Z","timestamp":1286232108000},"page":"18220-18225","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":429,"title":["Control of salicylic acid synthesis and systemic acquired resistance by two members of a plant-specific family of transcription factors"],"prefix":"10.1073","volume":"107","author":[{"given":"Yaxi","family":"Zhang","sequence":"first","affiliation":[{"name":"National Institute of Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, China;"},{"name":"College of Life Sciences, Beijing Normal University, Beijing 100875, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaohua","family":"Xu","sequence":"additional","affiliation":[{"name":"National Institute of Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, China;"},{"name":"Graduate Program, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pingtao","family":"Ding","sequence":"additional","affiliation":[{"name":"National Institute of Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, China;"},{"name":"College of Life Sciences, Beijing Normal University, Beijing 100875, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongmei","family":"Wang","sequence":"additional","affiliation":[{"name":"Michael Smith Laboratories, University of British Columbia, Vancouver, BC , Canada V6T 1Z4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu Ti","family":"Cheng","sequence":"additional","affiliation":[{"name":"Michael Smith Laboratories, University of British Columbia, Vancouver, BC , Canada V6T 1Z4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"He","sequence":"additional","affiliation":[{"name":"National Institute of Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minghui","family":"Gao","sequence":"additional","affiliation":[{"name":"National Institute of Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Xu","sequence":"additional","affiliation":[{"name":"Michael Smith Laboratories, University of British Columbia, Vancouver, BC , Canada V6T 1Z4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Li","sequence":"additional","affiliation":[{"name":"National Institute of Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaohai","family":"Zhu","sequence":"additional","affiliation":[{"name":"National Institute of Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Li","sequence":"additional","affiliation":[{"name":"Michael Smith Laboratories, University of British Columbia, Vancouver, BC , Canada V6T 1Z4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuelin","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Institute of Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2010,10,4]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.phyto.42.040803.140421"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2007.03067.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.phyto.050908.135202"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.250.4983.1002"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.250.4983.1004"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.97.4.1342"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(79)90019-9"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.2307\/3869297"},{"key":"e_1_3_3_9_2","first-page":"629","article-title":"Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat","volume":"8","author":"G\u00f6rlach J","year":"1996","unstructured":"J G\u00f6rlach, et al., Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat. Plant Cell 8, 629\u2013643 (1996).","journal-title":"Plant Cell"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.261.5122.754"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2004.07.005"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.014894"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00429-X"},{"key":"e_1_3_3_14_2","first-page":"1393","article-title":"Salicylic acid induction-deficient mutants of Arabidopsis express PR-2 and PR-5 and accumulate high levels of camalexin after pathogen inoculation","volume":"11","author":"Nawrath C","year":"1999","unstructured":"C Nawrath, JP M\u00e9traux, Salicylic acid induction-deficient mutants of Arabidopsis express PR-2 and PR-5 and accumulate high levels of camalexin after pathogen inoculation. Plant Cell 11, 1393\u20131404 (1999).","journal-title":"Plant Cell"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/35107108"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.108.065193"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2009.05.019"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111626200"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000301"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.2307\/3869945"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/143.2.973"},{"key":"e_1_3_3_22_2","first-page":"2033","article-title":"Characterization of eds1, a mutation in Arabidopsis suppressing resistance to Peronospora parasitica specified by several different RPP genes","volume":"8","author":"Parker JE","year":"1996","unstructured":"JE Parker, et al., Characterization of eds1, a mutation in Arabidopsis suppressing resistance to Peronospora parasitica specified by several different RPP genes. Plant Cell 8, 2033\u20132046 (1996).","journal-title":"Plant Cell"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81962-5"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.106.047688"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1005225107","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T18:24:22Z","timestamp":1649787862000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1005225107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,10,4]]},"references-count":24,"journal-issue":{"issue":"42","published-print":{"date-parts":[[2010,10,19]]}},"alternative-id":["10.1073\/pnas.1005225107"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1005225107","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,10,4]]},"assertion":[{"value":"2010-10-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}