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GTP or ITP functioned with less efficiency and adenosine 5\u03b3\u2032-(\u03b2,\u03b3-imido)triphosphate did not function at all. The\n                    <jats:italic>k<\/jats:italic>\n                    <jats:sub>cat<\/jats:sub>\n                    of eEF3 was 1.12\u00a0min\n                    <jats:sup>-1<\/jats:sup>\n                    , which is comparable to that of the in vitro initiation step. The disassembly reaction was inhibited by aminoglycosides and cycloheximide. The subunits formed from the yeast model PoTC remained separated under ionic conditions close to those existing in vivo, suggesting that they are ready to enter the initiation process. Based on our experimental techniques used in this paper, the release of mRNA and tRNA and ribosome dissociation took place simultaneously. No 40S\u2022mRNA complex was observed, indicating that eEF3 action promotes ribosome recycling, not reinitiation.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1006247107","type":"journal-article","created":{"date-parts":[[2010,6,1]],"date-time":"2010-06-01T20:51:16Z","timestamp":1275425476000},"page":"10854-10859","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":47,"title":["Ribosome recycling step in yeast cytoplasmic protein synthesis is catalyzed by eEF3 and ATP"],"prefix":"10.1073","volume":"107","author":[{"given":"Shinya","family":"Kurata","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA 19107;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus H.","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, University of Aarhus, 8000 \u212brhus C, 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