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Using\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    as a genetic model, we show that a key element to this coordination is the centrocone, a unique elaboration of the nuclear envelope that houses the mitotic spindle. Exploiting transgenic parasite lines expressing epitope-tagged centromeric H3 variant CenH3, we identify the centromeres of\n                    <jats:italic>T. gondii<\/jats:italic>\n                    chromosomes by hybridization of chromatin immunoprecipitations to genome-wide microarrays (ChIP-chip). We demonstrate that centromere attachment to the centrocone persists throughout the parasite cell cycle and that centromeres localize to a single apical region within the nucleus. Centromere sequestration provides a mechanism for the organization of the\n                    <jats:italic>Toxoplasma<\/jats:italic>\n                    nucleus and the maintenance of genome integrity.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1006741108","type":"journal-article","created":{"date-parts":[[2011,2,15]],"date-time":"2011-02-15T04:03:40Z","timestamp":1297742620000},"page":"3767-3772","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":114,"title":["<i>Toxoplasma gondii<\/i>\n                    sequesters centromeres to a specific nuclear region throughout the cell cycle"],"prefix":"10.1073","volume":"108","author":[{"given":"Carrie F.","family":"Brooks","sequence":"first","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria E.","family":"Francia","sequence":"additional","affiliation":[{"name":"Department of Cellular Biology, University of Georgia, Athens, GA 30602; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Gissot","sequence":"additional","affiliation":[{"name":"Departments of cMedicine and"},{"name":"Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew M.","family":"Croken","sequence":"additional","affiliation":[{"name":"Departments of cMedicine and"},{"name":"Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kami","family":"Kim","sequence":"additional","affiliation":[{"name":"Departments of cMedicine and"},{"name":"Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris","family":"Striepen","sequence":"additional","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases and"},{"name":"Department of Cellular Biology, University of Georgia, Athens, GA 30602; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2011,2,14]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1126\/science.1094717","article-title":"Intracellular parasite invasion strategies","volume":"304","author":"Sibley LD","year":"2004","unstructured":"LD Sibley, Intracellular parasite invasion strategies. 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