{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T22:24:05Z","timestamp":1792448645841,"version":"4.5.0"},"reference-count":36,"publisher":"National Academy of Sciences","issue":"31","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2012,7,31]]},"abstract":"<jats:p>\n                    The Gram-negative bacteria\n                    <jats:italic>Yersinia pestis<\/jats:italic>\n                    , causative agent of plague, is extremely virulent. One mechanism contributing to\n                    <jats:italic>Y. pestis<\/jats:italic>\n                    virulence is the presence of a type-three secretion system, which injects effector proteins, Yops, directly into immune cells of the infected host. One of these Yop proteins, YopJ, is proapoptotic and inhibits mammalian NF-\u03baB and MAP-kinase signal transduction pathways. Although the molecular mechanism remained elusive for some time, recent work has shown that YopJ acts as a serine\/threonine acetyl-transferase targeting MAP2 kinases. Using\n                    <jats:italic>Drosophila<\/jats:italic>\n                    as a model system, we find that YopJ inhibits one innate immune NF-\u03baB signaling pathway (IMD) but not the other (Toll). In fact, we show YopJ mediated serine\/threonine acetylation and inhibition of dTAK1, the critical MAP3 kinase in the IMD pathway. Acetylation of critical serine\/threonine residues in the activation loop of\n                    <jats:italic>Drosophila<\/jats:italic>\n                    TAK1 blocks phosphorylation of the protein and subsequent kinase activation. In addition, studies in mammalian cells show similar modification and inhibition of hTAK1. These data present evidence that TAK1 is a target for YopJ-mediated inhibition.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1008203109","type":"journal-article","created":{"date-parts":[[2012,7,17]],"date-time":"2012-07-17T09:21:03Z","timestamp":1342516863000},"page":"12710-12715","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":171,"title":["Serine\/threonine acetylation of TGF\u03b2-activated kinase (TAK1) by\n                    <i>Yersinia pestis<\/i>\n                    YopJ inhibits innate immune signaling"],"prefix":"10.1073","volume":"109","author":[{"given":"Nicholas","family":"Paquette","sequence":"first","affiliation":[{"name":"Division of Infectious Disease, Department of Medicine, and"},{"name":"Program of Developmental Immunology, Department of Pediatrics, Massachusetts General Hospital\/Harvard Medical School, Boston, MA 02114;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Conlon","sequence":"additional","affiliation":[{"name":"Division of Infectious Disease, Department of Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Sweet","sequence":"additional","affiliation":[{"name":"Division of Infectious Disease, Department of Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florentina","family":"Rus","sequence":"additional","affiliation":[{"name":"Division of Infectious Disease, Department of Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lindsay","family":"Wilson","sequence":"additional","affiliation":[{"name":"Division of Infectious Disease, Department of Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Pereira","sequence":"additional","affiliation":[{"name":"Division of Infectious Disease, Department of Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles V.","family":"Rosadini","sequence":"additional","affiliation":[{"name":"Division of Infectious Disease, Department of Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadege","family":"Goutagny","sequence":"additional","affiliation":[{"name":"Division of Infectious Disease, Department of Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander N. R.","family":"Weber","sequence":"additional","affiliation":[{"name":"Toll-Like Receptors and Cancer, German Cancer Research Center, Im Neuenheimer Feld 242, 69120 Heidelberg, Germany; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William S.","family":"Lane","sequence":"additional","affiliation":[{"name":"Mass Spectrometry and Proteomics Resource Laboratory, Center for Systems Biology, Harvard University, Cambridge, MA 02138"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott A.","family":"Shaffer","sequence":"additional","affiliation":[{"name":"Proteomics and Mass Spectrometry Facility, University of Massachusetts Medical School, Worcester, MA 01605;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Maniatis","sequence":"additional","affiliation":[{"name":"Proteomics and Mass Spectrometry Facility, University of Massachusetts Medical School, Worcester, MA 01605;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine A.","family":"Fitzgerald","sequence":"additional","affiliation":[{"name":"Division of Infectious Disease, Department of Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynda","family":"Stuart","sequence":"additional","affiliation":[{"name":"Program of Developmental Immunology, Department of Pediatrics, Massachusetts General Hospital\/Harvard Medical School, Boston, MA 02114;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neal","family":"Silverman","sequence":"additional","affiliation":[{"name":"Division of Infectious Disease, Department of Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2012,7,16]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.19.10385"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.11.2127"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00740.x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.2.708-716.1999"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00851.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.290.5496.1594"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00990.x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20051194"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608995103"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1126867"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.embor.7400754"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.12.8209"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.202396399"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80172-5"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(04)00104-9"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2009.12.036"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature08247"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2009.10.002"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M304802200"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0812022106"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(02)00325-8"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni955"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80627-3"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.10.7359"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M802825200"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M800943200"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M500646200"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M407537200"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.285.5435.1920"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000595"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni921"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2237236100"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2009.09.037"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2012.02.009"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.817800"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/35085597"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1008203109","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T20:04:30Z","timestamp":1649793870000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1008203109"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,7,16]]},"references-count":36,"journal-issue":{"issue":"31","published-print":{"date-parts":[[2012,7,31]]}},"alternative-id":["10.1073\/pnas.1008203109"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1008203109","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,7,16]]},"assertion":[{"value":"2012-07-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}