{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:08:02Z","timestamp":1790420882838,"version":"4.1.0"},"reference-count":25,"publisher":"National Academy of Sciences","issue":"33","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2010,8,17]]},"abstract":"<jats:p>\n                    The integral peroxisomal membrane proteins PEX10, PEX2, and PEX12 contain a zinc RING finger close to the C terminus. Loss of function of these peroxins causes embryo lethality at the heart stage in\n                    <jats:italic>Arabidopsis<\/jats:italic>\n                    . Preventing the coordination of Zn\n                    <jats:sup>2+<\/jats:sup>\n                    ions by amino acid substitutions in PEX10, PEX2, and PEX12 and overexpressing the resulting conditional sublethal mutations in WT uncovered additional functions of PEX10. Plants overexpressing \u0394Zn-mutant PEX10 display deformed peroxisomal shapes causing diminished contact with chloroplasts and possibly with mitochondria. These changes correlated with impaired metabolite transfer and, at high CO\n                    <jats:sub>2<\/jats:sub>\n                    , recoverable defective photorespiration plus dwarfish phenotype. The N-terminal PEX10 domain is critical for peroxisome biogenesis and plant development. A point mutation in the highly conserved TLGEEY motif results in vermiform peroxisome shape without impairing organelle contact. Addition of an N-terminal T7 tag to WT PEX0 resulted in partially recoverable reduced growth and defective inflorescences persisting under high CO\n                    <jats:sub>2<\/jats:sub>\n                    . In contrast, plants overexpressing PEX2-\u0394Zn-T7 grow like WT in normal atmosphere, contain normal-shaped peroxisomes, but display impaired peroxisomal matrix protein import. PEX12-\u0394Zn-T7 mutants exhibit unimpaired import of matrix protein and normal-shaped peroxisomes when grown in normal atmosphere. During seed germination, glyoxysomes form a reticulum around the lipid bodies for mobilization of storage oil. The formation of this glyoxysomal reticulum seemed to be impaired in PEX10-\u0394Zn but not in PEX2-\u0394Zn-T7 or PEX12-\u0394Zn-T7 plants. Both cytosolic PEX10 domains seem essential for peroxisome structure but differ in metabolic function, suggesting a role for this plant peroxin in addition to the import of matrix protein via ubiquitination of PEX5.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1009174107","type":"journal-article","created":{"date-parts":[[2010,8,2]],"date-time":"2010-08-02T23:35:49Z","timestamp":1280792149000},"page":"14915-14920","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":53,"title":["Different functions of the C\n                    <sub>3<\/sub>\n                    HC\n                    <sub>4<\/sub>\n                    zinc RING finger peroxins PEX10, PEX2, and PEX12 in peroxisome formation and matrix protein import"],"prefix":"10.1073","volume":"107","author":[{"given":"Jakob","family":"Prestele","sequence":"first","affiliation":[{"name":"LS Botanik and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg","family":"Hierl","sequence":"additional","affiliation":[{"name":"LS Botanik and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Scherling","sequence":"additional","affiliation":[{"name":"Bioinformatics and Biochemistry, Technische Universit\u00e4t Braunschweig, D-38106 Brunswick, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Hetkamp","sequence":"additional","affiliation":[{"name":"LS Botanik and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claus","family":"Schwechheimer","sequence":"additional","affiliation":[{"name":"LS Systembiologie der Pflanzen, Technische Universit\u00e4t M\u00fcnchen, D-85350 Freising, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika","family":"Isono","sequence":"additional","affiliation":[{"name":"LS Systembiologie der Pflanzen, Technische Universit\u00e4t M\u00fcnchen, D-85350 Freising, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfram","family":"Weckwerth","sequence":"additional","affiliation":[{"name":"Department of Molecular Systems Biology, University of Vienna, A-1090 Vienna, Austria; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard","family":"Wanner","sequence":"additional","affiliation":[{"name":"Ludwig-Maximilian-Universit\u00e4t M\u00fcnchen, Biozentrum, D-82152 Planegg-Martinsried, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Gietl","sequence":"additional","affiliation":[{"name":"LS Botanik and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2010,8,2]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1080\/09687680802130583"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.125.1.20"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2006.09.008"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2006.08.014"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0083-6729(05)72004-5"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10254-003-0007-z"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00678"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.104.052423"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/301963"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1633697100"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M403632200"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M413553200"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200611012"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.147.4.761"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M003303200"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1073633"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.103.031252"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.105.066811"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0610402104"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcf037"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.108.062265"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2009.03863.x"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.07.054"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2006.02809.x"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcm053"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1009174107","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T19:26:03Z","timestamp":1649791563000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1009174107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,8,2]]},"references-count":25,"journal-issue":{"issue":"33","published-print":{"date-parts":[[2010,8,17]]}},"alternative-id":["10.1073\/pnas.1009174107"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1009174107","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,8,2]]},"assertion":[{"value":"2010-08-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}