{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:25:44Z","timestamp":1791181544076,"version":"4.1.0"},"reference-count":39,"publisher":"National Academy of Sciences","issue":"43","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2010,10,26]]},"abstract":"<jats:p>\n                    Surface Ig (sIg) of follicular lymphoma (FL) is vital for tumor cell survival. We found previously that the Ig in FL is unusual, because the variable region genes carry sequence motifs for\n                    <jats:italic>N<\/jats:italic>\n                    -glycan addition. These are introduced by somatic mutation and are tumor specific. Unexpectedly, added glycans terminate at high mannose, suggesting a potentially important interaction of FL cells with mannose-binding lectins of the innate immune system. We have now identified mannosylated IgM at the surface of primary lymphoma cells. Recombinant lectin domains of the mannose receptor (MR) or DC-SIGN bind mannosylated Igs in vitro and bind to FL cells, signaling sIgM-associated increases in intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    . Lectins also bind to normal B cells but fail to signal. In contrast, anti-Ig signaled similarly in both FL and normal B cells. Mannosylation patterns were mimicked by FL Ig-derived single-chain Fvs (scFv), providing probes for potential receptors. Mannosylated scFv bound specifically to the lectin domains of the MR and DC-SIGN and blocked signaling. Mannosylated scFv also bound to DC-SIGN on the surface of dendritic cells. This unique lymphoma-specific interaction of sIg with lectins of innate immunity reveals a potential route for microenvironmental support of tumor cells, mediated via the key B-cell receptor.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1009388107","type":"journal-article","created":{"date-parts":[[2010,10,11]],"date-time":"2010-10-11T20:53:33Z","timestamp":1286830413000},"page":"18587-18592","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":174,"title":["Glycosylation of surface Ig creates a functional bridge between human follicular lymphoma and microenvironmental lectins"],"prefix":"10.1073","volume":"107","author":[{"given":"Vania","family":"Coelho","sequence":"first","affiliation":[{"name":"Molecular Immunology Group, Cancer Sciences Division, University of Southampton, School of Medicine, Southampton SO16 6YD, United Kingdom; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey","family":"Krysov","sequence":"additional","affiliation":[{"name":"Molecular Immunology Group, Cancer Sciences Division, University of Southampton, School of Medicine, Southampton SO16 6YD, United Kingdom; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amir M.","family":"Ghaemmaghami","sequence":"additional","affiliation":[{"name":"School of Molecular Medical Sciences, Institute of Infection, Immunity and Inflammation, Queen's Medical Centre, University of Nottingham, Nottingham NG7 2UH, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Emara","sequence":"additional","affiliation":[{"name":"School of Molecular Medical Sciences, Institute of Infection, Immunity and Inflammation, Queen's Medical Centre, University of Nottingham, Nottingham NG7 2UH, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen N.","family":"Potter","sequence":"additional","affiliation":[{"name":"Molecular Immunology Group, Cancer Sciences Division, University of Southampton, School of Medicine, Southampton SO16 6YD, United Kingdom; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Johnson","sequence":"additional","affiliation":[{"name":"Molecular Immunology Group, Cancer Sciences Division, University of Southampton, School of Medicine, Southampton SO16 6YD, United Kingdom; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graham","family":"Packham","sequence":"additional","affiliation":[{"name":"Molecular Immunology Group, Cancer Sciences Division, University of Southampton, School of Medicine, Southampton SO16 6YD, United Kingdom; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luisa","family":"Martinez-Pomares","sequence":"additional","affiliation":[{"name":"School of Molecular Medical Sciences, Institute of Infection, Immunity and Inflammation, Queen's Medical Centre, University of Nottingham, Nottingham NG7 2UH, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Freda K.","family":"Stevenson","sequence":"additional","affiliation":[{"name":"Molecular Immunology Group, Cancer Sciences Division, University of Southampton, School of Medicine, Southampton SO16 6YD, United Kingdom; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2010,10,11]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3874430"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V85.9.2528.bloodjournal8592528"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20082842"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.7.2562"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2003.04064.x"},{"key":"e_1_3_3_6_2","first-page":"541","article-title":"Acquired potential N-glycosylation sites within the tumor-specific immunoglobulin heavy chains of B-cell malignancies","volume":"89","author":"Zabalegui N","year":"2004","unstructured":"N Zabalegui, et al., Acquired potential N-glycosylation sites within the tumor-specific immunoglobulin heavy chains of B-cell malignancies. Haematologica 89, 541\u2013546 (2004).","journal-title":"Haematologica"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2007.10.009"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.3109\/10409239709085144"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M602690200"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-02-138933"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa041869"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-04-1565"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2007.12.8298"},{"key":"e_1_3_3_14_2","first-page":"1605","article-title":"The presence of STAT1-positive tumor-associated macrophages and their relation to outcome in patients with follicular lymphoma","volume":"91","author":"Alvaro T","year":"2006","unstructured":"T Alvaro, et al., The presence of STAT1-positive tumor-associated macrophages and their relation to outcome in patients with follicular lymphoma. Haematologica 91, 1605\u20131612 (2006).","journal-title":"Haematologica"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2008.00607.x"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-4128"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/immu.200310029"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2004.06.013"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1417"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.5.2089"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80373-6"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M104565200"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1066371"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830221046"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199911113412007"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.9.5489"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.2005.01693.x"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2008.02.003"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.3.1581"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.168.3.1099"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0905680"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(05)87005-1"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2007.10.005"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V65.6.1349.bloodjournal6561349"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-12-254847"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200106)31:6<1857::AID-IMMU1857>3.0.CO;2-D"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200535685"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-11-056648"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2007-8-3-r30"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1009388107","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T20:10:59Z","timestamp":1649794259000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1009388107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,10,11]]},"references-count":39,"journal-issue":{"issue":"43","published-print":{"date-parts":[[2010,10,26]]}},"alternative-id":["10.1073\/pnas.1009388107"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1009388107","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,10,11]]},"assertion":[{"value":"2010-10-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}