{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:43:55Z","timestamp":1781106235796,"version":"3.54.1"},"reference-count":44,"publisher":"National Academy of Sciences","issue":"1","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2011,1,4]]},"abstract":"<jats:p>\n                    Dynamic signals linking the actin cytoskeleton and cell adhesion receptors are essential for morphogenesis during development and normal tissue homeostasis. Abi1 is a central regulator of actin polymerization through interactions with multiple protein complexes. However, the in vivo role of Abi1 remains to be defined. The \u03b14 integrin adhesion receptor is associated with enhanced protrusive activity and regulation of directional cell migration. Among integrin subunits, \u03b14 exhibits unique properties in that it predominantly accumulates at the leading edge of migrating cells; however, the pathways that link the actin-regulatory machinery to \u03b14 at the leading edge have remained elusive. We generated Abi1 KO mice and found that loss of\n                    <jats:italic>Abi1<\/jats:italic>\n                    phenocopies KO of\n                    <jats:italic>\u03b14<\/jats:italic>\n                    . Mice lacking\n                    <jats:italic>Abi1<\/jats:italic>\n                    or\n                    <jats:italic>\u03b14<\/jats:italic>\n                    exhibit midgestational lethality with abnormalities in placental and cardiovascular development. Notably, purified Abi1 protein binds directly to the \u03b14 cytoplasmic tail and endogenous Abi1 colocalizes with phosphorylated \u03b14 at the leading edge of spreading cells. Moreover, Abi1-deficient cells expressing \u03b14 have impaired cell spreading, which is rescued by WT Abi1 but not an Abi1 mutant lacking the \u03b14-binding site. These data reveal a direct link between the \u03b14 integrin and actin polymerization and uncover a role for Abi1 in the regulation of morphogenesis in vivo. The Abi1\u2013\u03b14 interaction establishes a mechanistic paradigm for signaling between adhesion events and enhanced actin polymerization at the earliest stages of protrusion.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1012316108","type":"journal-article","created":{"date-parts":[[2010,12,20]],"date-time":"2010-12-20T23:23:52Z","timestamp":1292887432000},"page":"149-154","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":42,"title":["Abl-interactor-1 (Abi1) has a role in cardiovascular and placental development and is a binding partner of the \u03b14 integrin"],"prefix":"10.1073","volume":"108","author":[{"given":"Colleen","family":"Ring","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark H.","family":"Ginsberg","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California at San Diego, La Jolla, CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob","family":"Haling","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California at San Diego, La Jolla, CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann Marie","family":"Pendergast","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2010,12,20]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.041624"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/45264"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M102665200"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200304031"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.21.2569"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.21.2583"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2005.12.003"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.24.10905-10922.2004"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01483-08"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0050317"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2008.01.026"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1304"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2007.03.008"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2034"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.12.024"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.11.4979-4993.2004"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(01)00239-1"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2069"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2026"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.033324"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.121.2.549"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.121.2.489"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.1.1"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01770"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2006.06.014"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-10-051276"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI23445"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.125.22.4507"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.049551"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/35099066"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110928200"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1234"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0507072102"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02473"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2007.04.016"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.02-05-0086"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI26091"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.193.10.1149"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1050-1738(00)00073-6"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2008.00640.x"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2008.02.067"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI24751"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2353"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-09-3761"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1012316108","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T19:51:16Z","timestamp":1649793076000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1012316108"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,12,20]]},"references-count":44,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2011,1,4]]}},"alternative-id":["10.1073\/pnas.1012316108"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1012316108","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,12,20]]},"assertion":[{"value":"2010-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}