{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T22:54:26Z","timestamp":1791327266929,"version":"4.3.1"},"reference-count":43,"publisher":"National Academy of Sciences","issue":"4","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2011,1,25]]},"abstract":"<jats:p>\n                    Virulence of the intracellular pathogen\n                    <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    (\n                    <jats:italic>Listeria<\/jats:italic>\n                    ) requires escape from the phagosome into the host cytosol, where the bacteria replicate. Phagosomal escape is a multistep process characterized by perforation, which is dependent on the pore-forming toxin listeriolysin O (LLO), followed by rupture. The contribution of host factors to\n                    <jats:italic>Listeria<\/jats:italic>\n                    phagosomal escape is incompletely defined. Here we show that the cystic fibrosis transmembrane conductance regulator (CFTR) facilitates\n                    <jats:italic>Listeria<\/jats:italic>\n                    cytosolic entry. CFTR inhibition or mutation suppressed\n                    <jats:italic>Listeria<\/jats:italic>\n                    vacuolar escape in culture, and inhibition of CFTR in wild-type mice before oral inoculation of\n                    <jats:italic>Listeria<\/jats:italic>\n                    markedly decreased systemic infection. We provide evidence that high chloride concentrations may facilitate\n                    <jats:italic>Listeria<\/jats:italic>\n                    vacuolar escape by enhancing LLO oligomerization and lytic activity. We propose that CFTR transiently increases phagosomal chloride concentration after infection, potentiating LLO pore formation and vacuole lysis. Our studies suggest that\n                    <jats:italic>Listeria<\/jats:italic>\n                    exploits mechanisms of cellular ion homeostasis to escape the phagosome and emphasize host ion-channel function as a key parameter of bacterial virulence.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1013262108","type":"journal-article","created":{"date-parts":[[2011,1,10]],"date-time":"2011-01-10T23:55:41Z","timestamp":1294703741000},"page":"1633-1638","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":71,"title":["<i>Listeria monocytogenes<\/i>\n                    exploits cystic fibrosis transmembrane conductance regulator (CFTR) to escape the phagosome"],"prefix":"10.1073","volume":"108","author":[{"given":"Andrea L.","family":"Radtke","sequence":"first","affiliation":[{"name":"Departments of aMicrobiology and Immunology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelsi L.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Departments of aMicrobiology and Immunology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Davis","sequence":"additional","affiliation":[{"name":"Departments of aMicrobiology and Immunology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"DiMagno","sequence":"additional","affiliation":[{"name":"Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joel A.","family":"Swanson","sequence":"additional","affiliation":[{"name":"Departments of aMicrobiology and Immunology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary X.","family":"O'Riordan","sequence":"additional","affiliation":[{"name":"Departments of aMicrobiology and Immunology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2011,1,10]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200205009"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2005.00665.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2007.03.008"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00185-08"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.05.005"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.12.6754-6765.2003"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.10.6199-6209.2005"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0500558102"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07344"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0705801104"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.59.7.2082-2086.1993"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002320001053"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20080029"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0216112"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e09-01-0061"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M705296200"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1456"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.7.1159"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.22.12088"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/30006"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.75.103004.142532"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.05.001"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(01)02452-0"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00473.2003"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00405.2006"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.200409059"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1006\/prep.1998.1022"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi000436r"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.092160899"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac0521706"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1009655"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2002.32372"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.8.4848-4858.2004"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.04.048"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg194"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-11-61"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00018.2009"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.11.006"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2008.01199.x"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118253"},{"key":"e_1_3_3_41_2","first-page":"L625","article-title":"Dietary changes improve survival of CFTR S489X homozygous mutant mouse","volume":"269","author":"Eckman EA","year":"1995","unstructured":"EA Eckman, CU Cotton, DM Kube, PB Davis, Dietary changes improve survival of CFTR S489X homozygous mutant mouse. Am J Physiol 269, L625\u2013L630 (1995).","journal-title":"Am J Physiol"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3280353"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200201081"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1013262108","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T19:43:04Z","timestamp":1649792584000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1013262108"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,1,10]]},"references-count":43,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2011,1,25]]}},"alternative-id":["10.1073\/pnas.1013262108"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1013262108","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.7855956.8205055","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.7855956.8461057","asserted-by":"object"}]},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,1,10]]},"assertion":[{"value":"2011-01-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}