{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:52:52Z","timestamp":1791348772048,"version":"4.3.1"},"reference-count":47,"publisher":"National Academy of Sciences","issue":"7","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2011,2,15]]},"abstract":"<jats:p>\n                    OmpU porins are increasingly recognized as key determinants of pathogenic host\n                    <jats:italic>Vibrio<\/jats:italic>\n                    interactions. Although mechanisms remain incompletely understood, various species, including the human pathogen\n                    <jats:italic>Vibrio cholera<\/jats:italic>\n                    , require OmpU for host colonization and virulence. We have shown previously that OmpU is essential for virulence in the oyster pathogen\n                    <jats:italic>Vibrio splendidus<\/jats:italic>\n                    LGP32. Here, we showed that\n                    <jats:italic>V. splendidus<\/jats:italic>\n                    LGP32 invades the oyster immune cells, the hemocytes, through subversion of host-cell actin cytoskeleton. In this process, OmpU serves as an adhesin\/invasin required for \u03b2-integrin recognition and host cell invasion. Furthermore, the major protein of oyster plasma, the extracellular superoxide dismutase\n                    <jats:italic>Cg<\/jats:italic>\n                    -EcSOD, is used as an opsonin mediating the OmpU-promoted phagocytosis through its RGD sequence. Finally, the endocytosed bacteria were found to survive intracellularly, evading the host defense by preventing acidic vacuole formation and limiting reactive oxygen species production. We conclude that (\n                    <jats:italic>i<\/jats:italic>\n                    )\n                    <jats:italic>V. splendidus<\/jats:italic>\n                    is a facultative intracellular pathogen that manipulates host defense mechanisms to enter and survive in host immune cells, and (\n                    <jats:italic>ii<\/jats:italic>\n                    ) that OmpU is a major determinant of host cell invasion in\n                    <jats:italic>Vibrio<\/jats:italic>\n                    species, used by\n                    <jats:italic>V. splendidus<\/jats:italic>\n                    LGP32 to attach and invade oyster hemocytes through opsonisation by the oyster plasma\n                    <jats:italic>Cg<\/jats:italic>\n                    -EcSOD.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1015326108","type":"journal-article","created":{"date-parts":[[2011,1,31]],"date-time":"2011-01-31T23:07:07Z","timestamp":1296515227000},"page":"2993-2998","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":175,"title":["Use of OmpU porins for attachment and invasion of\n                    <i>Crassostrea gigas<\/i>\n                    immune cells by the oyster pathogen\n                    <i>Vibrio splendidus<\/i>"],"prefix":"10.1073","volume":"108","author":[{"given":"Marylise","family":"Duperthuy","sequence":"first","affiliation":[{"name":"Institut Fran\u00e7ais de Recherche pour l'Exploitation de la Mer,"},{"name":"Centre National de la Recherche Scientifique,"},{"name":"Universit\u00e9 Montpellier 2, and Institut de Recherche pour le D\u00e9veloppement, Laboratoire Ecosyst\u00e8mes Lagunaires, Unit\u00e9 Mixte de Recherche 5119, 34095 Montpellier, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulina","family":"Schmitt","sequence":"additional","affiliation":[{"name":"Institut Fran\u00e7ais de Recherche pour l'Exploitation de la Mer,"},{"name":"Centre National de la Recherche Scientifique,"},{"name":"Universit\u00e9 Montpellier 2, and Institut de Recherche pour le D\u00e9veloppement, Laboratoire Ecosyst\u00e8mes Lagunaires, Unit\u00e9 Mixte de Recherche 5119, 34095 Montpellier, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin","family":"Garz\u00f3n","sequence":"additional","affiliation":[{"name":"Institut de Recherche pour le D\u00e9veloppement, Centre de Coop\u00e9ration Internationale de la Recherche Agronomique pour le D\u00e9veloppement, Unit\u00e9 Mixte de Recherche 177, Interactions H\u00f4tes-Vecteurs-Parasites dans les Maladies dues aux Trypanosomatidae, 34094 Montpellier, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey","family":"Caro","sequence":"additional","affiliation":[{"name":"Institut Fran\u00e7ais de Recherche pour l'Exploitation de la Mer,"},{"name":"Centre National de la Recherche Scientifique,"},{"name":"Universit\u00e9 Montpellier 2, and Institut de Recherche pour le D\u00e9veloppement, Laboratoire Ecosyst\u00e8mes Lagunaires, Unit\u00e9 Mixte de Recherche 5119, 34095 Montpellier, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael D.","family":"Rosa","sequence":"additional","affiliation":[{"name":"Institut Fran\u00e7ais de Recherche pour l'Exploitation de la Mer,"},{"name":"Centre National de la Recherche Scientifique,"},{"name":"Universit\u00e9 Montpellier 2, and Institut de Recherche pour le D\u00e9veloppement, Laboratoire Ecosyst\u00e8mes Lagunaires, Unit\u00e9 Mixte de Recherche 5119, 34095 Montpellier, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9rique","family":"Le Roux","sequence":"additional","affiliation":[{"name":"Station Biologique de Roscoff, 29682 Roscoff, France;"},{"name":"Institut Fran\u00e7ais de Recherche pour l'Exploitation de la Mer, Laboratoire de Physiologie des Invert\u00e9br\u00e9s, 29280 Brest, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Lautr\u00e9dou-Audouy","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique and Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U554, Unit\u00e9 Mixte de Recherche 5048, Centre de Biochimie Structurale, 34090 Montpellier, France; 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