{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:22:19Z","timestamp":1791300139130,"version":"4.1.0"},"reference-count":25,"publisher":"National Academy of Sciences","issue":"6","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2011,2,8]]},"abstract":"<jats:p>\n                    Most strains of\n                    <jats:italic>Vibrio cholerae<\/jats:italic>\n                    are not pathogenic or cause only local outbreaks of gastroenteritis. Acquisition of the capacity to produce the cholera toxin results from a lysogenic conversion event due to a filamentous bacteriophage, CTX\u0278. Two\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    tyrosine recombinases that normally serve to resolve chromosome dimers, XerC and XerD, promote CTX\u0278 integration by directly recombining the ssDNA genome of the phage with the dimer resolution site of either or both\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    chromosomes. This smart mechanism renders the process irreversible. Many other filamentous vibriophages seem to attach to chromosome dimer resolution sites and participate in the rapid and continuous evolution of toxigenic\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    strains. We analyzed the molecular mechanism of integration of VGJ\u0278, a representative of the largest family of these phages. We found that XerC and XerD promote the integration of VGJ\u0278 into a specific chromosome dimer resolution site, and that the dsDNA replicative form of the phage is recombined. We show that XerC and XerD can promote excision of the integrated prophage, and that this participates in the production of new extrachromosomal copies of the phage genome. We further show how hybrid molecules harboring the concatenated genomes of CTX\u0278 and VGJ\u0278 can be produced efficiently. Finally, we discuss how the integration and excision mechanisms of VGJ\u0278 can explain the origin of recent epidemic\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    strains.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1017061108","type":"journal-article","created":{"date-parts":[[2011,1,25]],"date-time":"2011-01-25T01:09:22Z","timestamp":1295917762000},"page":"2516-2521","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":66,"title":["VGJ\u0278 integration and excision mechanisms contribute to the genetic diversity of\n                    <i>Vibrio cholerae<\/i>\n                    epidemic strains"],"prefix":"10.1073","volume":"108","author":[{"given":"Bhabatosh","family":"Das","sequence":"first","affiliation":[{"name":"Centre National de la Recherche Scientifique, Centre de G\u00e9n\u00e9tique Mol\u00e9culaire, 91198 Gif-sur-Yvette, France; and"},{"name":"Universit\u00e9 Paris-Sud, 91405 Orsay, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Bischerour","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique, Centre de G\u00e9n\u00e9tique Mol\u00e9culaire, 91198 Gif-sur-Yvette, France; and"},{"name":"Universit\u00e9 Paris-Sud, 91405 Orsay, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois-Xavier","family":"Barre","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique, Centre de G\u00e9n\u00e9tique Mol\u00e9culaire, 91198 Gif-sur-Yvette, France; and"},{"name":"Universit\u00e9 Paris-Sud, 91405 Orsay, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2011,1,24]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2003.08.004"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0907787106"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/1831533a0"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5270.1910"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature00782"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000201"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04309.x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0910212107"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2005.07.002"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02517.x"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2010.00937.x"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.032235-0"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0237385100"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.24.7231-7240.2003"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.19.5685-5696.2003"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(02)00005-X"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2004.11.046"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0700365104"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09469"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2004.02.003"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00793-8"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2004.09.007"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409900102"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.19.5530-5538.2000"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2008.01200.x"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1017061108","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T20:19:15Z","timestamp":1649794755000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1017061108"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,1,24]]},"references-count":25,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2011,2,8]]}},"alternative-id":["10.1073\/pnas.1017061108"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1017061108","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,1,24]]},"assertion":[{"value":"2011-01-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}