{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:10:20Z","timestamp":1791274220835,"version":"4.1.0"},"reference-count":60,"publisher":"National Academy of Sciences","issue":"30","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2011,7,26]]},"abstract":"<jats:p>Increased meiotic spindle abnormalities and aneuploidy in oocytes of women of advanced maternal ages lead to elevated rates of infertility, miscarriage, and trisomic conceptions. Despite the significance of the problem, strategies to sustain oocyte quality with age have remained elusive. Here we report that adult female mice maintained under 40% caloric restriction (CR) did not exhibit aging-related increases in oocyte aneuploidy, chromosomal misalignment on the metaphase plate, meiotic spindle abnormalities, or mitochondrial dysfunction (aggregation, impaired ATP production), all of which occurred in oocytes of age-matched ad libitum-fed controls. The effects of CR on oocyte quality in aging females were reproduced by deletion of the metabolic regulator, peroxisome proliferator-activated receptor \u03b3 coactivator-1\u03b1 (PGC-1\u03b1). Thus, CR during adulthood or loss of PGC-1\u03b1 function maintains female germline chromosomal stability and its proper segregation during meiosis, such that ovulated oocytes of aged female mice previously maintained on CR or lacking PGC-1\u03b1 are comparable to those of young females during prime reproductive life.<\/jats:p>","DOI":"10.1073\/pnas.1018793108","type":"journal-article","created":{"date-parts":[[2011,7,5]],"date-time":"2011-07-05T23:40:37Z","timestamp":1309909237000},"page":"12319-12324","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":182,"title":["Prevention of maternal aging-associated oocyte aneuploidy and meiotic spindle defects in mice by dietary and genetic strategies"],"prefix":"10.1073","volume":"108","author":[{"given":"Kaisa","family":"Selesniemi","sequence":"first","affiliation":[{"name":"Vincent Center for Reproductive Biology, Vincent Department of Obstetrics and Gynecology, Massachusetts General Hospital\/Harvard Medical School, Boston, MA 02114; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ho-Joon","family":"Lee","sequence":"additional","affiliation":[{"name":"Vincent Center for Reproductive Biology, Vincent Department of Obstetrics and Gynecology, Massachusetts General Hospital\/Harvard Medical School, Boston, MA 02114; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ailene","family":"Muhlhauser","sequence":"additional","affiliation":[{"name":"Center for Reproductive Biology and School of Molecular Biosciences, Washington State University, Pullman, WA 99164"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan L.","family":"Tilly","sequence":"additional","affiliation":[{"name":"Vincent Center for Reproductive Biology, Vincent Department of Obstetrics and Gynecology, Massachusetts General Hospital\/Harvard Medical School, Boston, MA 02114; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2011,7,5]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/218022a0"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00389450"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a019080"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2007.11.010"},{"key":"e_1_3_3_5_2","first-page":"1","article-title":"Trends and variations in first births to older women, United States, 1970-86","volume":"47","author":"Ventura SJ","year":"1989","unstructured":"SJ Ventura, Trends and variations in first births to older women, United States, 1970-86. Vital Health Stat 21 47, 1\u201327 (1989).","journal-title":"Vital Health Stat 21"},{"key":"e_1_3_3_6_2","first-page":"1","article-title":"Delayed childbearing: More women are having their first child later in life","volume":"21","author":"Matthews TJ","year":"2009","unstructured":"TJ Matthews, BE Hamilton, Delayed childbearing: More women are having their first child later in life. NCHS Data Brief 21, 1\u20138 (2009).","journal-title":"NCHS Data Brief"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod65.1.141"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.01.048"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.109.077909"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.10041"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(02)00636-2"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/geronj\/11.3.298"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/HUMREP\/10.6.1563"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cr.7310095"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a135954"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2005.03.012"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.77.061206.171059"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1172539"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0047-6374(94)90104-X"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1568-1637(02)00008-9"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1017\/S1464793103006213"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2005.03.019"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.11.034"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2009.03.003"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03354"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2005.05.004"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.09.013"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2005.03.002"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0030101"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-9726.2008.00409.x"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/60.12.1494"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2009.04.005"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0510452103"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1992.03490100073030"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1053\/beog.2002.0288"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)57665-1"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.288.18.2320"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1022580024402"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1097\/MOP.0b013e328332c6ab"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00302361"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2010.08.023"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/13.1.154"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/17.10.2678"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/35093050"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1117728"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2009.07.988"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.109.080887"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI42020"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/58.3.B212"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1173635"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-9726.2006.00202.x"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2060"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.2174\/1874609810801030145"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.18632\/aging.100034"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1002\/bies.200900171"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/54.11.B492"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1038\/80442"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1159\/000129914"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.108.074815"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng575"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1018793108","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T20:11:28Z","timestamp":1649794288000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1018793108"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,7,5]]},"references-count":60,"journal-issue":{"issue":"30","published-print":{"date-parts":[[2011,7,26]]}},"alternative-id":["10.1073\/pnas.1018793108"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1018793108","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.12911957.14779149","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.12911957.14201056","asserted-by":"object"}]},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,7,5]]},"assertion":[{"value":"2011-07-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}