{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:36:40Z","timestamp":1792669000136,"version":"4.5.0"},"reference-count":47,"publisher":"National Academy of Sciences","issue":"6","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2011,2,8]]},"abstract":"<jats:p>\n                    Improved protein-based vaccines should facilitate the goal of effective vaccines against HIV and other pathogens. With respect to T cells, the efficiency of immunization, or \u201cimmunogenicity,\u201d is improved by targeting vaccine proteins to maturing dendritic cells (DCs) within mAbs to DC receptors. Here, we compared the capacity of Langerin\/CD207, DEC205\/CD205, and Clec9A receptors, each expressed on the CD8\n                    <jats:sup>+<\/jats:sup>\n                    DC subset in mice, to bring about immunization of microbial-specific T cells from the polyclonal repertoire, using HIV gag-p24 protein as an antigen. \u03b1-Langerin mAb targeted splenic CD8\n                    <jats:sup>+<\/jats:sup>\n                    DCs selectively in vivo, whereas \u03b1-DEC205 and \u03b1-Clec9A mAbs targeted additional cell types. When the mAb heavy chains were engineered to express gag-p24, the \u03b1-Langerin, \u03b1-DEC205, and \u03b1-Clec9A fusion mAbs given along with a maturation stimulus induced comparable levels of gag-specific T helper 1 (Th1) and CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells in BALB\/c \u00d7 C57BL\/6 F1 mice. These immune T cells were more numerous than targeting the CD8\n                    <jats:sup>\u2212<\/jats:sup>\n                    DC subset with \u03b1-DCIR2-gag-p24. In an in vivo assay in which gag-primed T cells were used to report the early stages of T-cell responses, \u03b1-Langerin, \u03b1-DEC205, and \u03b1-Clec9A also mediated cross-presentation to primed CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells if, in parallel to antigen uptake, the DCs were stimulated with \u03b1-CD40. \u03b1-Langerin, \u03b1-DEC205, and \u03b1-Clec9A targeting greatly enhanced T-cell immunization relative to nonbinding control mAb or nontargeted HIV gag-p24 protein. Therefore, when the appropriate subset of DCs is targeted with a vaccine protein, several different receptors expressed by that subset are able to initiate combined Th1 and CD8\n                    <jats:sup>+<\/jats:sup>\n                    immunity.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1019547108","type":"journal-article","created":{"date-parts":[[2011,1,25]],"date-time":"2011-01-25T01:09:22Z","timestamp":1295917762000},"page":"2384-2389","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":247,"title":["Comparable T helper 1 (Th1) and CD8 T-cell immunity by targeting HIV gag p24 to CD8 dendritic cells within antibodies to Langerin, DEC205, and Clec9A"],"prefix":"10.1073","volume":"108","author":[{"given":"Juliana","family":"Idoyaga","sequence":"first","affiliation":[{"name":"Laboratory of Cellular Physiology and Immunology and The Chris Browne Center for Immunology and Immune Diseases, The Rockefeller University, New York, NY 10065;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashira","family":"Lubkin","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Physiology and Immunology and The Chris Browne Center for Immunology and Immune Diseases, The Rockefeller University, New York, NY 10065;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Fiorese","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Physiology and Immunology and The Chris Browne Center for Immunology and Immune Diseases, The Rockefeller University, New York, NY 10065;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mireille H.","family":"Lahoud","sequence":"additional","affiliation":[{"name":"The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia;"},{"name":"Departments of cMedical Biology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Caminschi","sequence":"additional","affiliation":[{"name":"The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia;"},{"name":"Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaoxing","family":"Huang","sequence":"additional","affiliation":[{"name":"Aaron Diamond AIDS Research Center, New York, NY 10016; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Physiology and Immunology and The Chris Browne Center for Immunology and Immune Diseases, The Rockefeller University, New York, NY 10065;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bj\u00f6rn E.","family":"Clausen","sequence":"additional","affiliation":[{"name":"Department of Immunology, Erasmus MC, University Medical Center, 3015 GE, Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chae Gyu","family":"Park","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Physiology and Immunology and The Chris Browne Center for Immunology and Immune Diseases, The Rockefeller University, New York, NY 10065;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Trumpfheller","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Physiology and Immunology and The Chris Browne Center for Immunology and Immune Diseases, The Rockefeller University, New York, NY 10065;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralph M.","family":"Steinman","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Physiology and Immunology and The Chris Browne Center for Immunology and Immune Diseases, The Rockefeller University, New York, NY 10065;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2011,1,24]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00290-09"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.6.2666-2673.2004"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200839035"},{"key":"e_1_3_3_4_2","first-page":"327","volume-title":"New Generation Vaccines","author":"Boscardin SB","year":"2009","unstructured":"SB Boscardin, C Trumpfheller, MC Nussenzweig, RM Steinman, Vaccines based on dendritic cell biology. New Generation Vaccines, ed MM Levine (USA: Informa Healthcare, New York), pp. 327\u2013339 (2009)."},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20032220"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20052005"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0610383104"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200939748"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1996"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri2103"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2009.00870.x"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.1.372"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1136080"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20070176"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201040511"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.11.1819"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.9.5448"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20020161"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20021215"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.9.5327"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.192.12.1685"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20020295"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20021598"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0601956103"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri723"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.2.741"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0812247106"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-05-155176"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI34584"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M709923200"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.6.3647"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1006\/cimm.1995.1109"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2007.05.001"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2007.02785.x"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.1.161"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.163.4.981"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/375151a0"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.194.6.769"},{"key":"e_1_3_3_39_2","doi-asserted-by":"crossref","first-page":"2985","DOI":"10.4049\/jimmunol.161.6.2985","article-title":"The MHC class I-restricted immune response to HIV-gag in BALB\/c mice selects a single epitope that does not have a predictable MHC-binding motif and binds to Kd through interactions between a glutamine at P3 and pocket D","volume":"161","author":"Mata M","year":"1998","unstructured":"M Mata, PJ Travers, Q Liu, FR Frankel, Y Paterson, The MHC class I-restricted immune response to HIV-gag in BALB\/c mice selects a single epitope that does not have a predictable MHC-binding motif and binds to Kd through interactions between a glutamine at P3 and pocket D. 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