{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T22:05:12Z","timestamp":1791065112324,"version":"4.1.0"},"reference-count":45,"publisher":"National Academy of Sciences","issue":"10","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2003,5,13]]},"abstract":"<jats:p>\n                    Gfi-1 is a zinc finger transcriptional repressor originally recognized for its role in T cell differentiation and lymphomas. Recent experiments reveal that gene-targeted\n                    <jats:italic>Gfi-1<\/jats:italic>\n                    -deficient mice are neutropenic and that\n                    <jats:italic>Gfi-1<\/jats:italic>\n                    mutations cause human neutropenia. In both cases, myeloid progenitor cells lose the ability to distinctly differentiate granulocytes from monocytes. The molecular mechanism of the hematopoietic abnormalities caused by\n                    <jats:italic>Gfi-1<\/jats:italic>\n                    deficiency remains undetermined because of a lack of known Gfi-1 target genes. To identify Gfi-1 targets\n                    <jats:italic>in vivo<\/jats:italic>\n                    , we performed large-scale chromatin immunoprecipitation analysis on a set of 34 candidate genes in myeloblast (KG-1 and HL-60), monoblast (U937), and T lymphocyte cell lines (Jurkat), in concert with RT-PCR-based expression profiling. We identified 32 Gfi-1 binding sites in a functionally variable set of 16 genes, including complements of cell-cycle regulators, transcription factors, and granulocyte-specific markers. Cluster analysis of expression patterns and chromatin immunoprecipitation data reveals that Gfi-1 targets a subset of genes differentiating hematopoietic lineages and therefore plays a relatively superior role in the hierarchy of factors governing stem cell differentiation.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1031694100","type":"journal-article","created":{"date-parts":[[2003,5,13]],"date-time":"2003-05-13T14:28:41Z","timestamp":1052836121000},"page":"5932-5937","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":86,"title":["Targets of the transcriptional repressor oncoprotein Gfi-1"],"prefix":"10.1073","volume":"100","author":[{"given":"Zhijun","family":"Duan","sequence":"first","affiliation":[{"name":"Division of Medical Genetics, Department of Medicine, University of Washington School of Medicine, 1705 Northeast Pacific Street, HSB-K236B, P.O. Box 357720, Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marshall","family":"Horwitz","sequence":"additional","affiliation":[{"name":"Division of Medical Genetics, Department of Medicine, University of Washington School of Medicine, 1705 Northeast Pacific Street, HSB-K236B, P.O. 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