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Using immunofluorescence, we now show that MYO7A and sans, a MYO7A-interacting protein, cluster at the UTLD. Analysis of the immunofluorescence intensity indicates that eight or more MYO7A molecules are present at each UTLD, consistent with a direct role for MYO7A in maintaining tip-link tension. MYO7A and sans localization at the UTLD is confirmed by transfection of hair cells with GFP-tagged constructs for these proteins. Cotransfection studies in a heterologous system show that MYO7A, sans, and the UTLD protein harmonin-b form a tripartite complex and that each protein is capable of interacting with one another independently. We propose that MYO7A, sans, and harmonin-b form the core components of the UTLD molecular complex. In this complex, MYO7A is likely the motor element that pulls on CDH23 to exert tension on the tip-link.<\/jats:p>","DOI":"10.1073\/pnas.1104161108","type":"journal-article","created":{"date-parts":[[2011,6,28]],"date-time":"2011-06-28T05:22:51Z","timestamp":1309238571000},"page":"11476-11481","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":205,"title":["Myosin VIIa and sans localization at stereocilia upper tip-link density implicates these Usher syndrome proteins in mechanotransduction"],"prefix":"10.1073","volume":"108","author":[{"given":"M\u2019hamed","family":"Grati","sequence":"first","affiliation":[{"name":"Laboratory of Cell Structure and Dynamics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bechara","family":"Kachar","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Structure and Dynamics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2011,6,27]]},"reference":[{"key":"e_1_3_3_1_2","first-page":"pt5","article-title":"The mechanotransduction machinery of hair cells","volume":"2","author":"Grillet N","year":"2009","unstructured":"N Grillet, et al., The mechanotransduction machinery of hair cells. 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