{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:59:24Z","timestamp":1791179964581,"version":"4.1.0"},"reference-count":32,"publisher":"National Academy of Sciences","issue":"19","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2011,5,10]]},"abstract":"<jats:p>\n                    The mosquito's body temperature increases dramatically when it takes a blood meal from a warm-blooded, vertebrate host. By using the yellow fever mosquito,\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    , we demonstrate that this boost in temperature following a blood meal prompts the synthesis of heat shock protein 70 (Hsp70). This response, elicited by the temperature of the blood meal, is most robust in the mosquito's midgut. When RNA interference is used to suppress expression of\n                    <jats:italic>hsp70<\/jats:italic>\n                    , protein digestion of the blood meal is impaired, leading to production of fewer eggs. We propose that Hsp70 protects the mosquito midgut from the temperature stress incurred by drinking a hot blood meal. Similar increases in\n                    <jats:italic>hsp70<\/jats:italic>\n                    were documented immediately after blood feeding in two other mosquitoes (\n                    <jats:italic>Culex pipiens<\/jats:italic>\n                    and\n                    <jats:italic>Anopheles gambiae<\/jats:italic>\n                    ) and the bed bug,\n                    <jats:italic>Cimex lectularius<\/jats:italic>\n                    , suggesting that this is a common protective response in blood-feeding arthropods.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1105195108","type":"journal-article","created":{"date-parts":[[2011,4,25]],"date-time":"2011-04-25T23:05:55Z","timestamp":1303772755000},"page":"8026-8029","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":158,"title":["Drinking a hot blood meal elicits a protective heat shock response in mosquitoes"],"prefix":"10.1073","volume":"108","author":[{"given":"Joshua B.","family":"Benoit","sequence":"first","affiliation":[{"name":"Departments of Entomology and Evolution, Ecology and Organismal Biology, The Ohio State University, Columbus, OH 43210;"},{"name":"Division of Epidemiology of Microbial Diseases, School of Public Health, Yale University, New Haven, CT 06510; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giancarlo","family":"Lopez-Martinez","sequence":"additional","affiliation":[{"name":"Departments of Entomology and Evolution, Ecology and Organismal Biology, The Ohio State University, Columbus, OH 43210;"},{"name":"Department of Entomology and Nematology, University of Florida, Gainesville, FL 32611"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin R.","family":"Patrick","sequence":"additional","affiliation":[{"name":"Departments of Entomology and Evolution, Ecology and Organismal Biology, The Ohio State University, Columbus, OH 43210;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachary P.","family":"Phillips","sequence":"additional","affiliation":[{"name":"Departments of Entomology and Evolution, Ecology and Organismal Biology, The Ohio State University, Columbus, OH 43210;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tyler B.","family":"Krause","sequence":"additional","affiliation":[{"name":"Departments of Entomology and Evolution, Ecology and Organismal Biology, The Ohio State University, Columbus, OH 43210;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David L.","family":"Denlinger","sequence":"additional","affiliation":[{"name":"Departments of Entomology and Evolution, Ecology and Organismal Biology, The Ohio State University, Columbus, OH 43210;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2011,4,25]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9780511610493"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00689629"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/arch.940070202"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.00639"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2010.02.014"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.61.1.243"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1379\/CSC-128R1.1"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1603\/033.046.0313"},{"key":"e_1_3_3_9_2","volume-title":"Hemolymph Proteins and Functional Peptides: Recent Advances in Insects and Other Arthropods","author":"Benoit JB","year":"2011","unstructured":"JB Benoit, G Lopez-Martinez Hemolymph Proteins and Functional Peptides: Recent Advances in Insects and Other Arthropods, eds M Tufail, M Takeda (Bentham, Oak Park, IL, 2011)."},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2009.09.012"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(03)00124-3"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-6-5"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.2005.00567.x"},{"key":"e_1_3_3_14_2","volume-title":"The Hot-Blooded Insects: Strategies and Mechanisms of Thermoregulation","author":"Heinrich B","year":"1993","unstructured":"B Heinrich The Hot-Blooded Insects: Strategies and Mechanisms of Thermoregulation (Harvard Univ Press, Cambridge, MA, 1993)."},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9780511675997"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00348047"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.2004.00537.x"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-552"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(03)00080-8"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0013359"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.200.14.2007"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2435.2001.00525.x"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0703538104"},{"key":"e_1_3_3_24_2","first-page":"312","article-title":"Insect cold tolerance and repair of chill-injury at fluctuating thermal regimes: Role of 70 kDa heat shock protein expression","volume":"30","author":"Tollarov\u00e1-Borovansk\u00e1 M","year":"2009","unstructured":"M Tollarov\u00e1-Borovansk\u00e1, L Lalouette, V Kost\u00e1l, Insect cold tolerance and repair of chill-injury at fluctuating thermal regimes: Role of 70 kDa heat shock protein expression. 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