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IgE- but not IgG-mediated murine anaphylaxis was accompanied by large increases in IL-4 secretion, plasma soluble IL-4 receptor-\u03b1 (IL-4R\u03b1) concentration, and T-cell membrane IL-4R\u03b1 expression. T-cell IL-4R\u03b1 expression also increased when mice that express human Fc\u03b5 receptor I\u03b1 were sensitized with IgG-depleted serum from a peanut-allergic individual and challenged with peanut extract. Increased T-cell IL-4R\u03b1 expression is likely to also be a marker for human IgE-mediated anaphylaxis, because IgE-activated human basophils secrete IL-4, and IL-4 increases human T-cell IL-4R\u03b1 expression in vitro. Murine IgG- but not IgE-mediated anaphylaxis was characterized by decreased neutrophil Fc\u03b3 receptor III (Fc\u03b3RIII) expression that was observed even when the antigen dose was insufficient to induce shock. Human neutrophils cultured with IgG immune complexes also lost Fc\u03b3RIII. These observations suggest that decreased blood neutrophil Fc\u03b3RIII expression without increased IL-4R\u03b1 expression can be used to determine whether and when IgG-mediated anaphylaxis occurs in man.<\/jats:p>","DOI":"10.1073\/pnas.1105695108","type":"journal-article","created":{"date-parts":[[2011,7,12]],"date-time":"2011-07-12T02:10:10Z","timestamp":1310436610000},"page":"12413-12418","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":140,"title":["Identification of markers that distinguish IgE- from IgG-mediated anaphylaxis"],"prefix":"10.1073","volume":"108","author":[{"given":"Marat V.","family":"Khodoun","sequence":"first","affiliation":[{"name":"Research Division, Cincinnati Veterans Affairs Medical Center, Cincinnati, OH 45220;"},{"name":"Division of Immunology, University of Cincinnati College of Medicine, Cincinnati, OH 45267; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Strait","sequence":"additional","affiliation":[{"name":"Research Division, Cincinnati Veterans Affairs Medical Center, Cincinnati, OH 45220;"},{"name":"Divisions of cEmergency Medicine and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Armstrong","sequence":"additional","affiliation":[{"name":"Research Division, Cincinnati Veterans Affairs Medical Center, Cincinnati, OH 45220;"},{"name":"Division of Immunology, University of Cincinnati College of Medicine, Cincinnati, OH 45267; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriko","family":"Yanase","sequence":"additional","affiliation":[{"name":"Research Division, Cincinnati Veterans Affairs Medical Center, Cincinnati, OH 45220;"},{"name":"Division of Immunology, University of Cincinnati College of Medicine, Cincinnati, OH 45267; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fred D.","family":"Finkelman","sequence":"additional","affiliation":[{"name":"Research Division, Cincinnati Veterans Affairs Medical Center, Cincinnati, OH 45220;"},{"name":"Division of Immunology, University of Cincinnati College of Medicine, Cincinnati, OH 45267; and"},{"name":"Immunobiology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2011,7,11]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.12.981"},{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1002\/0470861193.ch9","article-title":"Patterns of anaphylaxis: Acute and late phase features of allergic reactions","volume":"257","author":"Golden DB","year":"2004","unstructured":"DB Golden, Patterns of anaphylaxis: Acute and late phase features of allergic reactions. 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