{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:46:15Z","timestamp":1781102775128,"version":"3.54.1"},"reference-count":52,"publisher":"National Academy of Sciences","issue":"51","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2011,12,20]]},"abstract":"<jats:p>\n                    Rodents are natural reservoirs for a variety of species of\n                    <jats:italic>Borrelia<\/jats:italic>\n                    that cause relapsing fever (RF) in humans. The murine model of this disease recapitulates many of the clinical manifestations of the human disease and has revealed that T cell-independent antibody responses are required to resolve the bacteremic episodes. However, it is not clear whether such protective humoral responses are mounted in humans. We examined\n                    <jats:italic>Borrelia hermsii<\/jats:italic>\n                    infection in human hematopoietic stem cell-engrafted nonobese diabetic\/SCID\/IL-2R\u03b3\n                    <jats:sup>null<\/jats:sup>\n                    mice: \u201chuman immune system mice\u201d (HISmice). Infection of these mice, which are severely deficient in lymphoid and myeloid compartments, with\n                    <jats:italic>B. hermsii<\/jats:italic>\n                    resulted in persistent bacteremia. In contrast, this infection in HISmice resulted in recurrent episodes of bacteremia, the hallmark of RF. The resolution of the primary episode of bacteremia was concurrent with the generation of\n                    <jats:italic>B. hermsii<\/jats:italic>\n                    -specific human IgM. Remarkably, HISmice generated antibody responses to the\n                    <jats:italic>B. hermsii<\/jats:italic>\n                    outer-membrane protein Factor H binding protein A. Sera from humans infected by\n                    <jats:italic>B. hermsii<\/jats:italic>\n                    have a similar reactivity, and studies in mice have shown that this response is generated by the B1b cell subset. HISmice contain several B-cell subsets, including those with the phenotype CD20\n                    <jats:sup>+<\/jats:sup>\n                    CD27\n                    <jats:sup>+<\/jats:sup>\n                    CD43\n                    <jats:sup>+<\/jats:sup>\n                    CD70\n                    <jats:sup>\u2212<\/jats:sup>\n                    , a proposed human equivalent of mouse B1 cells. Reduction of B cells by administration of anti-human CD20 antibody resulted in diminished anti-\n                    <jats:italic>B. hermsii<\/jats:italic>\n                    responses and persistent bacteremia in HISmice. These data indicate that analysis of\n                    <jats:italic>B. hermsii<\/jats:italic>\n                    infection in HISmice will serve as a model in which to study the cellular and molecular mechanisms involved in controlling human RF.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1108776109","type":"journal-article","created":{"date-parts":[[2011,12,6]],"date-time":"2011-12-06T01:52:38Z","timestamp":1323136358000},"page":"20707-20712","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":35,"title":["Characteristics of\n                    <i>Borrelia hermsii<\/i>\n                    infection in human hematopoietic stem cell-engrafted mice mirror those of human relapsing fever"],"prefix":"10.1073","volume":"108","author":[{"given":"Raja","family":"Vuyyuru","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongqi","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim","family":"Manser","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kishore R.","family":"Alugupalli","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2011,12,5]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-196903000-00002"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/516276"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI5171"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.9.5832-5839.2001"},{"key":"e_1_3_3_5_2","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/B978-0-12-387050-6.50021-9","volume-title":"The Biology of Parasitic Spirochetes","author":"Burgdorfer W","year":"1976","unstructured":"W Burgdorfer, The epidemiology of the relapsing fevers. 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