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Here, we identified\n                    <jats:italic>mnoP<\/jats:italic>\n                    in the Gram-negative bacterium\n                    <jats:italic>Bradyrhizobium japonicum<\/jats:italic>\n                    as a gene coregulated with the inner membrane Mn\n                    <jats:sup>2+<\/jats:sup>\n                    transporter gene\n                    <jats:italic>mntH<\/jats:italic>\n                    . MnoP is an outer membrane protein expressed specifically under manganese limitation. MnoP acts as a channel to facilitate the tranlocation of Mn\n                    <jats:sup>2+<\/jats:sup>\n                    , but not Co\n                    <jats:sup>2+<\/jats:sup>\n                    or Cu\n                    <jats:sup>2+<\/jats:sup>\n                    , into reconstituted proteoliposomes. An\n                    <jats:italic>mnoP<\/jats:italic>\n                    mutant is defective in high-affinity Mn\n                    <jats:sup>2+<\/jats:sup>\n                    transport into cells and has a severe growth phenotype under manganese limitation. We suggest that the outer membrane is a barrier to divalent metal ions that requires a selective channel to meet the nutritional needs of the cell.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1110137108","type":"journal-article","created":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T07:38:57Z","timestamp":1314862737000},"page":"15390-15395","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":60,"title":["Bacterial outer membrane channel for divalent metal ion acquisition"],"prefix":"10.1073","volume":"108","author":[{"given":"Thomas H.","family":"Hohle","sequence":"first","affiliation":[{"name":"Department of Biochemistry, State University of New York, Buffalo, NY 14214; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William L.","family":"Franck","sequence":"additional","affiliation":[{"name":"National Center for Soybean Biotechnology, Division of Plant Sciences, University of Missouri, Columbia, MO 65211"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary","family":"Stacey","sequence":"additional","affiliation":[{"name":"National Center for Soybean Biotechnology, Division of Plant Sciences, University of Missouri, Columbia, MO 65211"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark R.","family":"O'Brian","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, State University of New York, Buffalo, NY 14214; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2011,8,31]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1093\/emboj\/20.5.935","article-title":"Ion channels in the outer membranes of chloroplasts and mitochondria: Open doors or regulated gates?","volume":"20","author":"B\u00f6lter B","year":"2001","unstructured":"B B\u00f6lter, J Soll, Ion channels in the outer membranes of chloroplasts and mitochondria: Open doors or regulated gates? 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