{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T07:30:44Z","timestamp":1793863844985,"version":"4.6.1"},"reference-count":30,"publisher":"National Academy of Sciences","issue":"49","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2011,12,6]]},"abstract":"<jats:p>\n                    <jats:italic>Vibrio cholerae<\/jats:italic>\n                    is an estuarine bacterium and the human pathogen responsible for the diarrheal disease cholera. In the environment, arthropods are proposed to be carriers and reservoirs of\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    . However, the molecular basis of the association between\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    and viable arthropods has not been elucidated previously. Here, we show that the\n                    <jats:italic>V. cholerae Vibrio<\/jats:italic>\n                    polysaccharide (VPS)-dependent biofilm is highly activated upon entry into the arthropod intestine and is specifically required for colonization of the arthropod rectum. Although the\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    VPS-dependent biofilm has been studied in the laboratory for many years, the function of this biofilm in the natural habitats of\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    has been elusive. Our results provide evidence that the VPS-dependent biofilm is required for intestinal colonization of an environmental host.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1111530108","type":"journal-article","created":{"date-parts":[[2011,11,22]],"date-time":"2011-11-22T02:22:31Z","timestamp":1321928551000},"page":"19737-19742","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":77,"title":["Spatially selective colonization of the arthropod intestine through activation of\n                    <i>Vibrio cholerae<\/i>\n                    biofilm formation"],"prefix":"10.1073","volume":"108","author":[{"given":"Alexandra E.","family":"Purdy","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Children's Hospital, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula I.","family":"Watnick","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Children's Hospital, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2011,11,21]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(07)70138-X"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2002.tb02659.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0008607"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2008.01753.x"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2006.01011.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2005.00745.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-706X(00)00162-5"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.46.1.32-36.1983"},{"key":"e_1_3_3_9_2","first-page":"23","article-title":"[Houseflies (M. domestica L.) as transmitters of the agent of cholera]","author":"Kotenok IaF","year":"1977","unstructured":"IaF Kotenok, IuV Chicherin, [Houseflies (M. domestica L.) as transmitters of the agent of cholera]. 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