{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T05:25:23Z","timestamp":1792733123136,"version":"4.5.0"},"reference-count":32,"publisher":"National Academy of Sciences","issue":"45","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2011,11,8]]},"abstract":"<jats:p>\n                    Unlike broad-host\n                    <jats:italic>Salmonella<\/jats:italic>\n                    serovars, which cause self-limiting disease,\n                    <jats:italic>Salmonella enterica<\/jats:italic>\n                    serovar Typhi can infect only humans causing typhoid fever, a life-threatening systemic disease. The molecular bases for these differences are presently unknown. Here we show that the GTPase Rab29 (Rab7L1) distinguishes the intracellular vacuole of human-adapted and broad-host\n                    <jats:italic>Salmonella<\/jats:italic>\n                    serovars. A screen to identify host factors required for the export of typhoid toxin, which is exclusively encoded by the human-specific\n                    <jats:italic>Salmonella enterica<\/jats:italic>\n                    serovars Typhi (\n                    <jats:italic>S<\/jats:italic>\n                    . Typhi) and Paratyphi (\n                    <jats:italic>S<\/jats:italic>\n                    . Paratyphi) identified Rab29. We found that Rab29 is recruited to the\n                    <jats:italic>S<\/jats:italic>\n                    . Typhi-containing vacuole but not to vacuoles containing broad-host\n                    <jats:italic>Salmonella<\/jats:italic>\n                    . We observed that in cells infected with broad-host\n                    <jats:italic>Salmonella<\/jats:italic>\n                    Rab29 is specifically cleaved by the proteolytic activity of GtgE, a unique type III secretion effector protein that is absent from\n                    <jats:italic>S<\/jats:italic>\n                    . Typhi. An\n                    <jats:italic>S<\/jats:italic>\n                    . Typhi strain engineered to express GtgE and therefore able to cleave Rab29 exhibited increased intracellular replication in human macrophages. These findings indicate significant differences in the intracellular biology of human-adapted and broad-host\n                    <jats:italic>Salmonella<\/jats:italic>\n                    and show how subtle differences in the assortment of effector proteins encoded by highly related pathogens can have a major impact in their biology.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1111959108","type":"journal-article","created":{"date-parts":[[2011,11,1]],"date-time":"2011-11-01T00:21:08Z","timestamp":1320106868000},"page":"18418-18423","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":118,"title":["Proteolytic targeting of Rab29 by an effector protein distinguishes the intracellular compartments of human-adapted and broad-host\n                    <i>Salmonella<\/i>"],"prefix":"10.1073","volume":"108","author":[{"given":"Stefania","family":"Span\u00f2","sequence":"first","affiliation":[{"name":"Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06536"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyun","family":"Liu","sequence":"additional","affiliation":[{"name":"Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06536"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge E.","family":"Gal\u00e1n","sequence":"additional","affiliation":[{"name":"Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06536"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2011,10,31]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(98)01236-0"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/649541"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra020201"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/318457"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2010.01904.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2007.11.001"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400932101"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2010.09.003"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-010-0436-1"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2728"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.16.4394"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200606050"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.17.1.53"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2003.00294.x"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2009.01368.x"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02230.x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200309161"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature04822"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.170128997"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00984.x"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03598.x"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.19.5234-5239.2002"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1470"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/35101607"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1039\/c0mb00097c"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80549-8"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2009.00942.x"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(93)90065-U"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.9.2901-2908.1991"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/291238a0"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb00450.x"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1201476"}],"container-title":["Proceedings 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