{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T04:46:15Z","timestamp":1789965975413,"version":"4.0.1"},"reference-count":41,"publisher":"National Academy of Sciences","issue":"4","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2012,1,24]]},"abstract":"<jats:p>\n                    The target-of-rapamycin pathway couples nutrient availability with tissue and organismal growth in metazoans. The key effectors underlying this growth are, however, unclear. Here we show that Maf1, a repressor of RNA polymerase III-dependent tRNA transcription, is an important mediator of nutrient-dependent growth in\n                    <jats:italic>Drosophila<\/jats:italic>\n                    . We find nutrients promote tRNA synthesis during larval development by inhibiting Maf1. Genetic inhibition of Maf1 accelerates development and increases body size. These phenotypes are due to a non\u2013cell-autonomous effect of Maf1 inhibition in the fat body, the main larval endocrine organ. Inhibiting Maf1 in the fat body increases growth by promoting the expression of brain-derived insulin-like peptides and consequently enhanced systemic insulin signaling. Remarkably, the effects of Maf1 inhibition are reproduced in flies carrying one extra copy of the initiator methionine tRNA, tRNA\n                    <jats:sub>i<\/jats:sub>\n                    <jats:sup>Met<\/jats:sup>\n                    . These findings suggest the stimulation of tRNA\n                    <jats:sub>i<\/jats:sub>\n                    <jats:sup>Met<\/jats:sup>\n                    synthesis via inhibition of dMaf1 is limiting for nutrition-dependent growth during development.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1113311109","type":"journal-article","created":{"date-parts":[[2012,1,7]],"date-time":"2012-01-07T00:35:23Z","timestamp":1325896523000},"page":"1139-1144","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":102,"title":["<i>Drosophila<\/i>\n                    RNA polymerase III repressor Maf1 controls body size and developmental timing by modulating tRNA\n                    <sub>i<\/sub>\n                    <sup>Met<\/sup>\n                    synthesis and systemic insulin signaling"],"prefix":"10.1073","volume":"109","author":[{"given":"Elizabeth J.","family":"Rideout","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Clark H. Smith Brain Tumour Centre, Southern Alberta Cancer Research Institute, University of Calgary, Calgary, AB, Canada T2N 4N1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynne","family":"Marshall","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Clark H. Smith Brain Tumour Centre, Southern Alberta Cancer Research Institute, University of Calgary, Calgary, AB, Canada T2N 4N1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Savraj S.","family":"Grewal","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Clark H. 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