{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:35:51Z","timestamp":1791200151537,"version":"4.2.0"},"reference-count":50,"publisher":"National Academy of Sciences","issue":"47","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2011,11,22]]},"abstract":"<jats:p>\n                    Negative-strand (NS) RNA viruses comprise many pathogens that cause serious diseases in humans and animals. Despite their clinical importance, little is known about the host factors required for their infection. Using vesicular stomatitis virus (VSV), a prototypic NS RNA virus in the family\n                    <jats:italic>Rhabdoviridae<\/jats:italic>\n                    , we conducted a human genome-wide siRNA screen and identified 72 host genes required for viral infection. Many of these identified genes were also required for infection by two other NS RNA viruses, the lymphocytic choriomeningitis virus of the\n                    <jats:italic>Arenaviridae<\/jats:italic>\n                    family and human parainfluenza virus type 3 of the\n                    <jats:italic>Paramyxoviridae<\/jats:italic>\n                    family. Genes affecting different stages of VSV infection, such as entry\/uncoating, gene expression, and assembly\/release, were identified. Depletion of the proteins of the coatomer complex I or its upstream effectors ARF1 or GBF1 led to detection of reduced levels of VSV RNA. Coatomer complex I was also required for infection of lymphocytic choriomeningitis virus and human parainfluenza virus type 3. These results highlight the evolutionarily conserved requirements for gene expression of diverse families of NS RNA viruses and demonstrate the involvement of host cell secretory pathway in the process.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1113643108","type":"journal-article","created":{"date-parts":[[2011,11,8]],"date-time":"2011-11-08T01:51:56Z","timestamp":1320717116000},"page":"19036-19041","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":86,"title":["RNAi screening reveals requirement for host cell secretory pathway in infection by diverse families of negative-strand RNA viruses"],"prefix":"10.1073","volume":"108","author":[{"given":"Debasis","family":"Panda","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine and Biomedical Sciences and"},{"name":"Nebraska Center for Virology, University of Nebraska, Lincoln, NE 68583;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anshuman","family":"Das","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Biomedical Sciences and"},{"name":"Nebraska Center for Virology, University of Nebraska, Lincoln, NE 68583;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phat X.","family":"Dinh","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Biomedical Sciences and"},{"name":"Nebraska Center for Virology, University of Nebraska, Lincoln, NE 68583;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sakthivel","family":"Subramaniam","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Biomedical Sciences and"},{"name":"Nebraska Center for Virology, University of Nebraska, Lincoln, NE 68583;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debasis","family":"Nayak","sequence":"additional","affiliation":[{"name":"National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas J.","family":"Barrows","sequence":"additional","affiliation":[{"name":"Duke RNAi Screening Facility, Duke University Medical Center, Durham, NC 27710;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James L.","family":"Pearson","sequence":"additional","affiliation":[{"name":"Duke RNAi Screening Facility, Duke University Medical Center, Durham, NC 27710;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesse","family":"Thompson","sequence":"additional","affiliation":[{"name":"Nebraska Center for Virology, University of Nebraska, Lincoln, NE 68583;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David L.","family":"Kelly","sequence":"additional","affiliation":[{"name":"University of Nebraska Medical Center, Omaha, NE 68198; 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