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This switch includes the ordering of a polypeptide stretch containing Tyr195, and major movement of an approximately 30-residue \u201clid\u201d segment covering the active site. The rearranged lid guides the nascent maltosaccharide chain into the active site in either an intra- or intersubunit mode dependent upon chain length and steric factors and positions the donor and acceptor sugar groups for catalysis. The Thr83Met mutation, which causes glycogen storage disease XV, is conformationally locked in the ground state and catalytically inactive. Our data highlight the conformational plasticity of glycogenin and coexistence of two modes of glucosylation as integral to its catalytic mechanism.<\/jats:p>","DOI":"10.1073\/pnas.1113921108","type":"journal-article","created":{"date-parts":[[2011,12,13]],"date-time":"2011-12-13T00:15:49Z","timestamp":1323735349000},"page":"21028-21033","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":52,"title":["Conformational plasticity of glycogenin and its maltosaccharide substrate during glycogen biogenesis"],"prefix":"10.1073","volume":"108","author":[{"given":"Apirat","family":"Chaikuad","sequence":"first","affiliation":[{"name":"Structural Genomics Consortium, Old Road Research Campus Building, Oxford, United Kingdom OX3 7DQ; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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