{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T20:27:23Z","timestamp":1792009643398,"version":"4.4.0"},"reference-count":41,"publisher":"National Academy of Sciences","issue":"11","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2003,5,27]]},"abstract":"<jats:p>\n                    Hypoxic\u2013ischemic brain injury in premature infants results in cerebral white matter lesions with prominent oligodendroglial injury and loss, a disorder termed periventricular leukomalacia (PVL). We have previously shown that glutamate receptors mediate hypoxic\u2013ischemic injury to oligodendroglial precursor cells (OPCs) in a model of PVL in the developing rodent brain. We used primary OPC cultures to examine the mechanism of cellular toxicity induced by oxygen\u2013glucose deprivation (OGD) to simulate brain ischemia. OPCs were more sensitive to OGD-induced toxicity than mature oligodendrocytes, and OPC toxicity was attenuated by nonselective [2,3-dihydroxy-6-nitro-7-sulfamoylbenzo[\n                    <jats:italic>f<\/jats:italic>\n                    ]quinoxaline (NBQX), 6-cyano-7-nitroquinoxaline-2,3-dione], \u03b1-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-preferring (GYKI 52466), kainate-preferring (\u03b3-\n                    <jats:sc>d<\/jats:sc>\n                    -glutamylaminomethanesulfonic acid), or Ca\n                    <jats:sup>2<\/jats:sup>\n                    <jats:sup>+<\/jats:sup>\n                    -permeable AMPA\/kainate receptor antagonists (joro spider toxin, JSTx) administered either during or after OGD. Furthermore, NBQX or JSTx blocked OGD-induced Ca\n                    <jats:sup>2<\/jats:sup>\n                    <jats:sup>+<\/jats:sup>\n                    influx. Relevant to recurrent hypoxic\u2013ischemic insults in developing white matter, we examined the effects of sublethal OGD preconditioning. A prior exposure of OPCs to sublethal OGD resulted in enhanced vulnerability to subsequent excitotoxic or OGD-induced injury associated with an increased Ca\n                    <jats:sup>2<\/jats:sup>\n                    <jats:sup>+<\/jats:sup>\n                    influx. AMPA\/kainate receptor blockade with NBQX or JSTx either during or after sublethal OGD prevented its priming effect. Furthermore, OGD preconditioning resulted in a down-regulation of the AMPA receptor subunit GluR2 on cell surface that increased Ca\n                    <jats:sup>2<\/jats:sup>\n                    <jats:sup>+<\/jats:sup>\n                    permeability of the receptors. Overall, these data suggest that aberrantly enhanced activation of Ca\n                    <jats:sup>2<\/jats:sup>\n                    <jats:sup>+<\/jats:sup>\n                    -permeable AMPA\/kainate receptors may be a major mechanism in acute and repeated hypoxic\u2013ischemic injury to OPCs in disorders of developing cerebral white matter, such as PVL.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1136624100","type":"journal-article","created":{"date-parts":[[2003,5,27]],"date-time":"2003-05-27T13:10:32Z","timestamp":1054041032000},"page":"6801-6806","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":177,"title":["Calcium-permeable AMPA\/kainate receptors mediate toxicity and preconditioning by oxygen-glucose deprivation in oligodendrocyte precursors"],"prefix":"10.1073","volume":"100","author":[{"given":"Wenbin","family":"Deng","sequence":"first","affiliation":[{"name":"Department of Neurology and Program in Neuroscience, Children's Hospital and Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul A.","family":"Rosenberg","sequence":"additional","affiliation":[{"name":"Department of Neurology and Program in Neuroscience, Children's Hospital and Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph J.","family":"Volpe","sequence":"additional","affiliation":[{"name":"Department of Neurology and Program in Neuroscience, Children's Hospital and Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frances E.","family":"Jensen","sequence":"additional","affiliation":[{"name":"Department of Neurology and Program in Neuroscience, Children's Hospital and Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2003,5,12]]},"reference":[{"key":"e_1_3_2_1_2","unstructured":"Volpe J. 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