{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T03:28:52Z","timestamp":1793244532533,"version":"4.5.0"},"reference-count":35,"publisher":"National Academy of Sciences","issue":"46","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2012,11,13]]},"abstract":"<jats:p>\n                    Although West Nile virus (WNV) and other arthropod-borne viruses are a major public health problem, the mechanisms of antiviral immunity in mosquitoes are poorly understood. Dicer-2, responsible for the RNAi-mediated response through the C-terminal RNase-III domain, also contains an N-terminal DExD\/H-box helicase domain similar to mammalian RIG-I\/MDA5 which, in\n                    <jats:italic>Drosophila<\/jats:italic>\n                    , was found to be required for activation of an antiviral gene,\n                    <jats:italic>Vago<\/jats:italic>\n                    . Here we show that the\n                    <jats:italic>Culex<\/jats:italic>\n                    orthologue of Vago (\n                    <jats:italic>Cx<\/jats:italic>\n                    Vago) is up-regulated in response to WNV infection in a Dicer-2\u2013dependent manner. Further, our data show that\n                    <jats:italic>Cx<\/jats:italic>\n                    Vago is a secreted peptide that restricts WNV infection by activation of the Jak-STAT pathway. Thus, Vago appears to function as an IFN-like antiviral cytokine in mosquitoes.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1205231109","type":"journal-article","created":{"date-parts":[[2012,10,2]],"date-time":"2012-10-02T02:37:49Z","timestamp":1349145469000},"page":"18915-18920","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":327,"title":["Secreted Vago restricts West Nile virus infection in\n                    <i>Culex<\/i>\n                    mosquito cells by activating the Jak-STAT pathway"],"prefix":"10.1073","volume":"109","author":[{"given":"Prasad N.","family":"Paradkar","sequence":"first","affiliation":[{"name":"CSIRO Animal, Food and Health Sciences, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee","family":"Trinidad","sequence":"additional","affiliation":[{"name":"CSIRO Animal, Food and Health Sciences, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rhonda","family":"Voysey","sequence":"additional","affiliation":[{"name":"CSIRO Animal, Food and Health Sciences, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Bernard","family":"Duchemin","sequence":"additional","affiliation":[{"name":"CSIRO Ecosystem Sciences, Australian Animal Health Laboratory, Geelong, VIC 3220 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J.","family":"Walker","sequence":"additional","affiliation":[{"name":"CSIRO Animal, Food and Health Sciences, 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