{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:26:28Z","timestamp":1791293188709,"version":"4.1.0"},"reference-count":23,"publisher":"National Academy of Sciences","issue":"42","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2012,10,16]]},"abstract":"<jats:p>\n                    Dysfunction of the neurotrophin brain-derived neurotrophic factor (BDNF) is implicated in Rett syndrome (RTT), but the state of its releasable pool and downstream signaling in mice lacking methyl-CpG-binding protein-2 (\n                    <jats:italic>Mecp2<\/jats:italic>\n                    ) is unknown. Here, we show that membrane currents and dendritic Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signals evoked by recombinant BDNF or an activator of diacylglycerol (DAG)-sensitive transient receptor potential canonical (TRPC) channels are impaired in CA3 pyramidal neurons of symptomatic\n                    <jats:italic>Mecp2<\/jats:italic>\n                    mutant mice. TRPC3 and TRPC6 mRNA and protein levels are lower in\n                    <jats:italic>Mecp2<\/jats:italic>\n                    mutant hippocampus, and chromatin immunoprecipitation (ChIP) identified\n                    <jats:italic>Trpc3<\/jats:italic>\n                    as a target of MeCP2 transcriptional regulation. BDNF mRNA and protein levels are also lower in\n                    <jats:italic>Mecp2<\/jats:italic>\n                    mutant hippocampus and dentate gyrus granule cells, which is reflected in impaired activity-dependent release of endogenous BDNF estimated from TRPC currents and dendritic Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signals in CA3 pyramidal neurons. These results identify the gene encoding TRPC3 channels as a MeCP2 target and suggest a potential therapeutic strategy to boost impaired BDNF signaling in RTT.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1205271109","type":"journal-article","created":{"date-parts":[[2012,10,2]],"date-time":"2012-10-02T02:37:49Z","timestamp":1349145469000},"page":"17087-17092","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":72,"title":["Activity-dependent BDNF release and TRPC signaling is impaired in hippocampal neurons of\n                    <i>Mecp2<\/i>\n                    mutant mice"],"prefix":"10.1073","volume":"109","author":[{"given":"Wei","family":"Li","sequence":"first","affiliation":[{"name":"Department of Neurobiology, Civitan International Research Center, The University of Alabama at Birmingham, Birmingham, AL 35294; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaston","family":"Calfa","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Civitan International Research Center, The University of Alabama at Birmingham, Birmingham, AL 35294; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Larimore","sequence":"additional","affiliation":[{"name":"Biology Department and Neuroscience Program, Agnes Scott College, Decatur, GA 30030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas","family":"Pozzo-Miller","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Civitan International Research Center, The University of Alabama at Birmingham, Birmingham, AL 35294; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2012,10]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/13810"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1153252"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/35049004"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5503-09.2010"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80838-7"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5499-06.2007"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.01140.2009"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.68.040204.100431"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/16711"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/85906"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00797.2007"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-005-1416-4"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M008104200"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3846-04.2004"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201201038"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1086446"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1090842"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1996.76.1.554"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03477"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn1870"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00800.2010"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0b013e3181d6b852"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09582"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1205271109","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T20:41:35Z","timestamp":1649796095000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1205271109"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10]]},"references-count":23,"journal-issue":{"issue":"42","published-print":{"date-parts":[[2012,10,16]]}},"alternative-id":["10.1073\/pnas.1205271109"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1205271109","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10]]},"assertion":[{"value":"2012-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}