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In this study, we report the unexpected finding that homologous chromosomes contact each other at the sites of DSBs induced by either radiation or the endonuclease I-PpoI in human somatic cells. Contact involves short segments of homologous chromosomes and is centered on a DSB in active genes but does not occur at I-PpoI sites in intergenic DNA. I-PpoI-induced contact between homologous genes is abrogated by the transcriptional inhibitors actinomycin D and \u03b1-amanitin and requires the kinase activity of ATM but not DNA-PK. Our findings provide documentation of a common transcription-related and ATM kinase-dependent mechanism that induces contact between allelic regions of homologous chromosomes at sites of DSBs in human somatic cells.<\/jats:p>","DOI":"10.1073\/pnas.1205759109","type":"journal-article","created":{"date-parts":[[2012,5,30]],"date-time":"2012-05-30T00:35:30Z","timestamp":1338338130000},"page":"9454-9459","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":50,"title":["Homologous chromosomes make contact at the sites of double-strand breaks in genes in somatic G\n                    <sub>0<\/sub>\n                    \/G\n                    <sub>1<\/sub>\n                    -phase human cells"],"prefix":"10.1073","volume":"109","author":[{"given":"Manoj","family":"Gandhi","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of Pittsburgh, Pittsburgh, PA 15213;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viktoria N.","family":"Evdokimova","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of Pittsburgh, Pittsburgh, PA 15213;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"T.Cuenco","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, School of Dental Medicine and"},{"name":"Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 15219;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina N.","family":"Nikiforova","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of Pittsburgh, Pittsburgh, PA 15213;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lindsey M.","family":"Kelly","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of Pittsburgh, Pittsburgh, PA 15213;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James R.","family":"Stringer","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, University of Cincinnati, Cincinnati, OH 45267; 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