{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T21:04:29Z","timestamp":1790975069369,"version":"4.1.0"},"reference-count":50,"publisher":"National Academy of Sciences","issue":"15","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2001,7,17]]},"abstract":"<jats:p>\n                    DNA polymerase \u03b7 (Pol\u03b7) functions in the error-free bypass of UV-induced DNA lesions, and a defect in Pol\u03b7 in humans causes the cancer-prone syndrome, the variant form of xeroderma pigmentosum. Both yeast and human Pol\u03b7 replicate through a cis-syn thymine-thymine dimer (TT dimer) by inserting two As opposite the two Ts of the dimer. Pol\u03b7, however, is a low-fidelity enzyme, and it misinserts nucleotides with a frequency of \u2248 10\n                    <jats:sup>\u22122<\/jats:sup>\n                    to 10\n                    <jats:sup>\u22123<\/jats:sup>\n                    opposite the two Ts of the TT dimer as well as opposite the undamaged template bases. This low fidelity of nucleotide insertion seems to conflict with the role of Pol\u03b7 in the error-free bypass of UV lesions. To resolve this issue, we have examined the ability of human and yeast Pol\u03b7 to extend from paired and mispaired primer termini opposite a TT dimer by using steady-state kinetic assays. We find that Pol\u03b7 extends from mispaired primer termini on damaged and undamaged DNAs with a frequency of \u2248 10\n                    <jats:sup>\u22122<\/jats:sup>\n                    to 10\n                    <jats:sup>\u22123<\/jats:sup>\n                    relative to paired primer termini. Thus, after the incorporation of an incorrect nucleotide, Pol\u03b7 would dissociate from the DNA rather than extend from the mispair. The resulting primer-terminal mispair then could be subject to proofreading by a 3\u2032\u21925\u2032 exonuclease. Replication through a TT dimer by Pol\u03b7 then would be more accurate than that predicted from the fidelity of nucleotide incorporation alone.\n                  <\/jats:p>","DOI":"10.1073\/pnas.121007298","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:34:10Z","timestamp":1027679650000},"page":"8355-8360","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":66,"title":["Accuracy of lesion bypass by yeast and human DNA polymerase \u03b7"],"prefix":"10.1073","volume":"98","author":[{"given":"M. Todd","family":"Washington","sequence":"first","affiliation":[{"name":"Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, TX 77555-1061"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert E.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, TX 77555-1061"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise","family":"Prakash","sequence":"additional","affiliation":[{"name":"Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, TX 77555-1061"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satya","family":"Prakash","sequence":"additional","affiliation":[{"name":"Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, TX 77555-1061"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2001,7,17]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.22.12224"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(76)90156-X"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(71)90204-X"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2660(08)60299-0"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ge.27.120193.000341"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.8.7.811"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.37.23360"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.12.9.3807-3818.1992"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/147.4.1557"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.23.15975"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)46922-0"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004380050698"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.283.5404.1001"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5268.1646"},{"key":"e_1_3_3_15_2","first-page":"21","volume":"28","author":"Lawrence C W","year":"1996","unstructured":"C W Lawrence, D C Hinkle Cancer Surv 28, 21\u201331 (1996).","journal-title":"Cancer Surv"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.19.3137"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/35023030"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.1.185-188.2001"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.72.1.219"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1159\/000131646"},{"key":"e_1_3_3_21_2","first-page":"2593","volume":"50","author":"Boyer J C","year":"1990","unstructured":"J C Boyer, W K Kaufmann, B P Brylawski, M Cordeiro-Stone Cancer Res 50, 2593\u20132598 (1990).","journal-title":"Cancer Res"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.13.7.4276-4283.1993"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12371686"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.285.5425.263"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/21447"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.52.36835"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.11.7447"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.7.3094"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/35010014"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M009049200"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.3109\/10409239309086792"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.9.4759"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)39981-8"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00216a030"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/20.17.4567"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C000690200"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.12.3100"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/78169"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.23.4717"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.21.8001-8007.2000"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.19.5259"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.10.3558-3563.2001"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1995.tb05236.x"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00219a034"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.3.719"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00200a006"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.7.1407"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00353a012"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00353a013"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.25.17395"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.121007298","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T20:49:22Z","timestamp":1649796562000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.121007298"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,7,17]]},"references-count":50,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2001,7,17]]}},"alternative-id":["10.1073\/pnas.121007298"],"URL":"https:\/\/doi.org\/10.1073\/pnas.121007298","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,7,17]]},"assertion":[{"value":"2001-07-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}