{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T17:00:35Z","timestamp":1781110835658,"version":"3.54.1"},"reference-count":20,"publisher":"National Academy of Sciences","issue":"6","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2013,2,5]]},"abstract":"<jats:p>\n                    Hematopoietic stem cells (HSCs) are the source of all blood lineages, and HSCs must balance quiescence, self-renewal, and differentiation to meet lifelong needs for blood cell development. Transformation of HSCs by the breakpoint cluster region-ABL tyrosine kinase (BCR-ABL) oncogene causes chronic myelogenous leukemia (CML). The E-twenty six (ets) transcription factor GA binding protein (GABP) is a tetrameric transcription factor complex that contains GABP\u03b1 and GABP\u03b2 proteins. Deletion in bone marrow of\n                    <jats:italic>Gabpa<\/jats:italic>\n                    , the gene that encodes the DNA-binding component, caused cell cycle arrest in HSCs and profound loss of hematopoietic progenitor cells. Loss of Gabp\u03b1 prevented development of CML, although mice continued to generate BCR-ABL\u2013expressing Gabp\u03b1-null cells for months that were serially transplantable and contributed to all lineages in secondary recipients. A bioinformatic screen identified the serine-threonine kinase protein kinase D2 (PRKD2) as a potential effector of GABP in HSCs.\n                    <jats:italic>Prkd2<\/jats:italic>\n                    expression was markedly reduced in Gabp\u03b1-null HSCs and progenitor cells. Reduced expression of PRKD2 or pharmacologic inhibition decreased cell cycling, and PRKD2 rescued growth of Gabp\u03b1-null BCR-ABL\u2013expressing cells. Thus, GABP is required for HSC cell cycle entry and CML development through its control of PRKD2. This offers a potential therapeutic target in leukemia.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1212904110","type":"journal-article","created":{"date-parts":[[2013,1,24]],"date-time":"2013-01-24T05:03:36Z","timestamp":1359003816000},"page":"2312-2317","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":27,"title":["GABP transcription factor is required for development of chronic myelogenous leukemia via its control of PRKD2"],"prefix":"10.1073","volume":"110","author":[{"given":"Zhong-Fa","family":"Yang","sequence":"first","affiliation":[{"name":"Division of Hematology\u2013Oncology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01655;"},{"name":"School of Basic Medical Sciences, TaiShan Medical University, TaiAn 271016, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haojian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Division of Hematology\u2013Oncology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01655;"},{"name":"Division of Genomic Stability and DNA Repair, Department of Radiation Oncology, Dana\u2013Farber Cancer Institute, Boston, MA 02215;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leyuan","family":"Ma","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01655;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong","family":"Peng","sequence":"additional","affiliation":[{"name":"Division of Hematology\u2013Oncology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01655;"},{"name":"Division of Hematology\/Oncology, Children\u2019s Hospital Boston, Boston, MA 02115;"},{"name":"Department of Pediatric Oncology, Dana\u2013Farber Cancer Institute, Boston, MA 02115; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaoyu","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of Hematology\u2013Oncology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01655;"},{"name":"Oncology, Novartis Institutes for BioMedical Research; Cambridge, MA 02139"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junling","family":"Wang","sequence":"additional","affiliation":[{"name":"Division of Hematology\u2013Oncology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01655;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Green","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01655;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoguang","family":"Li","sequence":"additional","affiliation":[{"name":"Division of Hematology\u2013Oncology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01655;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan G.","family":"Rosmarin","sequence":"additional","affiliation":[{"name":"Division of Hematology\u2013Oncology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01655;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2013,1,23]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.01.025"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa055229"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.189.9.1399"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcmd.2003.09.005"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1117"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2007.03.010"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.13.5844-5849.2004"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1548"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-07-298802"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-09-306563"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0606509103"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-06-228130"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.389"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0510423103"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00037.2010"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.219"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-09-1145"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M313225200"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-010-0016-z"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-10-387381"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1212904110","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T21:38:17Z","timestamp":1649799497000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1212904110"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1,23]]},"references-count":20,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2013,2,5]]}},"alternative-id":["10.1073\/pnas.1212904110"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1212904110","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,1,23]]},"assertion":[{"value":"2013-01-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}