{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T07:16:16Z","timestamp":1793344576637,"version":"4.5.0"},"reference-count":29,"publisher":"National Academy of Sciences","issue":"2","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2013,1,8]]},"abstract":"<jats:p>\n                    Research into the aetiological agent of the most widespread form of severe malaria,\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    , has benefitted enormously from the ability to culture and genetically manipulate blood-stage forms of the parasite in vitro. However, most malaria outside Africa is caused by a distinct\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    species,\n                    <jats:italic>Plasmodium vivax<\/jats:italic>\n                    , and it has become increasingly apparent that zoonotic infection by the closely related simian parasite\n                    <jats:italic>Plasmodium knowlesi<\/jats:italic>\n                    is a frequent cause of life-threatening malaria in regions of southeast Asia. Neither of these important malarial species can be cultured in human cells in vitro, requiring access to primates with the associated ethical and practical constraints. We report the successful adaptation of\n                    <jats:italic>P. knowlesi<\/jats:italic>\n                    to continuous culture in human erythrocytes. Human-adapted\n                    <jats:italic>P. knowlesi<\/jats:italic>\n                    clones maintain their capacity to replicate in monkey erythrocytes and can be genetically modified with unprecedented efficiency, providing an important and unique model for studying conserved aspects of malarial biology as well as species-specific features of an emerging pathogen.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1216457110","type":"journal-article","created":{"date-parts":[[2012,12,24]],"date-time":"2012-12-24T23:14:38Z","timestamp":1356390878000},"page":"531-536","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":313,"title":["Adaptation of the genetically tractable malaria pathogen\n                    <i>Plasmodium knowlesi<\/i>\n                    to continuous culture in human erythrocytes"],"prefix":"10.1073","volume":"110","author":[{"given":"Robert W.","family":"Moon","sequence":"first","affiliation":[{"name":"Division of Parasitology, Medical Research Council National Institute for Medical Research, London NW7 1AA, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Hall","sequence":"additional","affiliation":[{"name":"Division of Retrovirology, National Institute for Biological Standards and Control, Health Protection Agency, Hertfordshire EN6 3QG, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farania","family":"Rangkuti","sequence":"additional","affiliation":[{"name":"Pathogen Genomics Laboratory, Computational Bioscience Research Center, King Abdullah University of Science and Technology, Thuwal-Jeddah, Saudi Arabia; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yung Shwen","family":"Ho","sequence":"additional","affiliation":[{"name":"Pathogen Genomics Laboratory, Computational Bioscience Research Center, King Abdullah University of Science and Technology, Thuwal-Jeddah, Saudi Arabia; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil","family":"Almond","sequence":"additional","affiliation":[{"name":"Division of Retrovirology, National Institute for Biological Standards and Control, Health Protection Agency, Hertfordshire EN6 3QG, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graham H.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Peter Gorer Department of Immunobiology, King\u2019s College London School of Medicine at Guy\u2019s, King\u2019s and St Thomas\u2019 Hospitals, Guy\u2019s Hospital, London SE1 9RT, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnab","family":"Pain","sequence":"additional","affiliation":[{"name":"Pathogen Genomics Laboratory, Computational Bioscience Research Center, King Abdullah University of Science and Technology, Thuwal-Jeddah, Saudi Arabia; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony A.","family":"Holder","sequence":"additional","affiliation":[{"name":"Division of Parasitology, Medical Research Council National Institute for Medical Research, London NW7 1AA, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Blackman","sequence":"additional","affiliation":[{"name":"Division of Parasitology, Medical Research Council National Institute for Medical Research, London NW7 1AA, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2012,12,24]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1126\/science.781840","article-title":"Human malaria parasites in continuous culture","volume":"193","author":"Trager W","year":"1976","unstructured":"W Trager, JB Jensen, Human malaria parasites in continuous culture. 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