{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:54:33Z","timestamp":1781103273786,"version":"3.54.1"},"reference-count":44,"publisher":"National Academy of Sciences","issue":"12","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2002,6,11]]},"abstract":"<jats:p>\n                    The efficiency of gene transfer into human hematopoietic stem cells by oncoretroviral vectors is too low for effective gene therapy of most hematologic diseases. Retroviral vectors based on the nonpathogenic foamy viruses (FV) are an alternative gene-transfer system. In this study, human umbilical cord blood CD34\n                    <jats:sup>+<\/jats:sup>\n                    cells were transduced with FV vectors by a single 10-h exposure to vector stocks and then injected into sublethally irradiated nonobese diabetic-severe combined immunodeficiency (NOD\/SCID) mice. At 5\u20137 weeks after transplantation, high transgene expression rates were observed in engrafted human hematopoietic cells, including over 60% of clonogenic progenitors. Significant transgene silencing did not occur. We developed an approach for expanding human cell populations derived from transplanted mice to show that multiple SCID repopulating cells (SRCs) had been transduced, including some that were capable of both lymphoid and myeloid differentiation. These findings demonstrate for the first time that human pluripotent (lympho-myeloid) hematopoietic stem cells repopulate NOD\/SCID mice and can be efficiently transduced by FV vectors.\n                  <\/jats:p>","DOI":"10.1073\/pnas.122131099","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:46:49Z","timestamp":1027680409000},"page":"8295-8300","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":80,"title":["Transduction of human NOD\/SCID-repopulating cells with both lymphoid and myeloid potential by foamy virus vectors"],"prefix":"10.1073","volume":"99","author":[{"given":"Neil C.","family":"Josephson","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Hematology, and Department of Laboratory Medicine, University of Washington, Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Vassilopoulos","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Hematology, and Department of Laboratory Medicine, University of Washington, Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grant D.","family":"Trobridge","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Hematology, and Department of Laboratory Medicine, University of Washington, Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greg V.","family":"Priestley","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Hematology, and Department of Laboratory Medicine, University of Washington, Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brent L.","family":"Wood","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Hematology, and Department of Laboratory Medicine, University of Washington, Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thalia","family":"Papayannopoulou","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Hematology, and Department of Laboratory Medicine, University of Washington, Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Russell","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Hematology, and Department of Laboratory Medicine, University of Washington, Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2002,6,11]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.3.1747-1755.1999"},{"key":"e_1_3_3_2_2","first-page":"739","volume":"4","author":"Flugel R M","year":"1991","unstructured":"R M Flugel J Acquired Immune Defic Syndr 4, 739\u2013750 (1991).","journal-title":"J Acquired Immune Defic Syndr"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1089\/aid.1996.12.1473"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1089\/aid.1995.11.161"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0498-403"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)05292-7"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.1.217-222.1996"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1089\/10430349850019355"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.3.604"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/00062752-199603060-00002"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/stem.160166"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1296-1329"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.10.5320"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V89.12.4307"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02633976"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V93.11.3736.411k01_3736_3749"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(02)46082-X"},{"key":"e_1_3_3_18_2","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook J","year":"1989","unstructured":"J Sambrook, E F Fritsch, T Maniatis Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Lab. 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