{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T12:30:17Z","timestamp":1790512217551,"version":"4.1.0"},"reference-count":32,"publisher":"National Academy of Sciences","issue":"24","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2013,6,11]]},"abstract":"<jats:p>The thymus provides multiple microenvironments that are essential for the development and repertoire selection of T lymphocytes. The thymic cortex induces the generation and positive selection of T lymphocytes, whereas the thymic medulla establishes self-tolerance among the positively selected T lymphocytes. Cortical thymic epithelial cells (cTECs) and medullary TECs (mTECs) constitute the major stromal cells that structurally form and functionally characterize the cortex and the medulla, respectively. cTECs and mTECs are both derived from the endodermal epithelium of the third pharyngeal pouch. However, the molecular and cellular characteristics of the progenitor cells for the distinct TEC lineages are unclear. Here we report the preparation and characterization of mice that express the recombinase Cre instead of \u03b25t, a proteasome subunit that is abundant in cTECs and not detected in other cell types, including mTECs. By crossing \u03b25t-Cre knock-in mice with loxP-dependent GFP reporter mice, we found that \u03b25t-Cre\u2013mediated recombination occurs specifically in TECs but not in any other cell types in the mouse. Surprisingly, in addition to cTECs, \u03b25t-Cre-loxP\u2013mediated GFP expression was detected in almost all mTECs. These results indicate that the majority of mTECs, including autoimmune regulator-expressing mTECs, are derived from \u03b25t-expressing progenitor cells.<\/jats:p>","DOI":"10.1073\/pnas.1301799110","type":"journal-article","created":{"date-parts":[[2013,5,30]],"date-time":"2013-05-30T01:03:13Z","timestamp":1369875793000},"page":"9885-9890","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":142,"title":["Aire-expressing thymic medullary epithelial cells originate from \u03b25t-expressing progenitor cells"],"prefix":"10.1073","volume":"110","author":[{"given":"Izumi","family":"Ohigashi","sequence":"first","affiliation":[{"name":"Division of Experimental Immunology, Institute for Genome Research, University of Tokushima, Tokushima 770-8503, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saulius","family":"Zuklys","sequence":"additional","affiliation":[{"name":"Laboratory of Pediatric Immunology, Department of Biomedicine, University of Basel and University Children's Hospital Basel, 4058 Basel, Switzerland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mie","family":"Sakata","sequence":"additional","affiliation":[{"name":"Division of Experimental Immunology, Institute for Genome Research, University of Tokushima, Tokushima 770-8503, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos E.","family":"Mayer","sequence":"additional","affiliation":[{"name":"Laboratory of Pediatric Immunology, Department of Biomedicine, University of Basel and University Children's Hospital Basel, 4058 Basel, Switzerland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saule","family":"Zhanybekova","sequence":"additional","affiliation":[{"name":"Laboratory of Pediatric Immunology, Department of Biomedicine, University of Basel and University Children's Hospital Basel, 4058 Basel, Switzerland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeo","family":"Murata","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo 113-0033, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiji","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506, Japan; 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