{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T12:45:19Z","timestamp":1792759519565,"version":"4.5.0"},"reference-count":53,"publisher":"National Academy of Sciences","issue":"35","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2013,8,27]]},"abstract":"<jats:p>\n                    Type I fibrillar collagen is the most abundant protein in the human body, crucial for the formation and strength of bones, skin, and tendon. Proteolytic enzymes are essential for initiation of the assembly of collagen fibrils by cleaving off the propeptides. We report that\n                    <jats:italic>\n                      Mep1a\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    and\n                    <jats:italic>Mep1b<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice revealed lower amounts of mature collagen I compared with WT mice and exhibited significantly reduced collagen deposition in skin, along with markedly decreased tissue tensile strength. While exploring the mechanism of this phenotype, we found that cleavage of full-length human procollagen I heterotrimers by either meprin \u03b1 or meprin \u03b2 led to the generation of mature collagen molecules that spontaneously assembled into collagen fibrils. Thus, meprin \u03b1 and meprin \u03b2 are unique in their ability to process and release both C- and N-propeptides from type I procollagen in vitro and in vivo and contribute to the integrity of connective tissue in skin, with consequent implications for inherited connective tissue disorders.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1305464110","type":"journal-article","created":{"date-parts":[[2013,8,12]],"date-time":"2013-08-12T21:53:00Z","timestamp":1376344380000},"page":"14219-14224","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":143,"title":["Metalloproteases meprin \u03b1 and meprin \u03b2 are C- and N-procollagen proteinases important for collagen assembly and tensile strength"],"prefix":"10.1073","volume":"110","author":[{"given":"Claudia","family":"Broder","sequence":"first","affiliation":[{"name":"Unit for Degradomics of the Protease Web, Institute of Biochemistry, University of Kiel, 24118 Kiel, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Arnold","sequence":"additional","affiliation":[{"name":"Institute of Zoology, Johannes Gutenberg University, 55128 Mainz, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandrine","family":"Vadon-Le Goff","sequence":"additional","affiliation":[{"name":"Tissue Biology and Therapeutic Engineering Unit, Centre National de la Recherche Scientifique\/University of Lyon, Unit\u00e9 Mixte de Recherche 5305, Unit\u00e9 Mixte de Service 3444 Biosciences Gerland-Lyon Sud, 69367 Lyon Cedex 7, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moritz A.","family":"Konerding","sequence":"additional","affiliation":[{"name":"Institute of Functional and Clinical Anatomy, University Medical Center, Johannes Gutenberg University, 55128 Mainz, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerstin","family":"Bahr","sequence":"additional","affiliation":[{"name":"Institute of Functional and Clinical Anatomy, University Medical Center, Johannes Gutenberg University, 55128 Mainz, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, University of Bern, CH-3010 Bern, Switzerland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher M.","family":"Overall","sequence":"additional","affiliation":[{"name":"Centre for Blood Research, University of British Columbia, Vancouver, BC, Canada V6T 1Z3;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith S.","family":"Bond","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, PA 17033; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomas","family":"Koudelka","sequence":"additional","affiliation":[{"name":"Institute of Experimental Medicine, University of Kiel, 24118 Kiel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Tholey","sequence":"additional","affiliation":[{"name":"Institute of Experimental Medicine, University of Kiel, 24118 Kiel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J. S.","family":"Hulmes","sequence":"additional","affiliation":[{"name":"Tissue Biology and Therapeutic Engineering Unit, Centre National de la Recherche Scientifique\/University of Lyon, Unit\u00e9 Mixte de Recherche 5305, Unit\u00e9 Mixte de Service 3444 Biosciences Gerland-Lyon Sud, 69367 Lyon Cedex 7, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Moali","sequence":"additional","affiliation":[{"name":"Tissue Biology and Therapeutic Engineering Unit, Centre National de la Recherche Scientifique\/University of Lyon, Unit\u00e9 Mixte de Recherche 5305, Unit\u00e9 Mixte de Service 3444 Biosciences Gerland-Lyon Sud, 69367 Lyon Cedex 7, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Becker-Pauly","sequence":"additional","affiliation":[{"name":"Unit for Degradomics of the Protease Web, Institute of Biochemistry, University of Kiel, 24118 Kiel, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2013,8,12]]},"reference":[{"key":"e_1_3_3_1_2","volume-title":"Roles and Regulation of BMP-1\/Tolloid-Like Proteinases: Collagen\/Matrix Assembly, Growth Factor Activation and Beyond","author":"Moali C","year":"2012","unstructured":"C Moali, DJS Hulmes, Extracellular Matrix: Pathobiology and Signaling. Roles and Regulation of BMP-1\/Tolloid-Like Proteinases: Collagen\/Matrix Assembly, Growth Factor Activation and Beyond, ed M Karamanos (De Gruyter, Berlin, 2012)."},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-73906-9_2"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.2002.4450"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(00)00082-2"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.01731"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1208072109"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M105601200"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2007.05.004"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M501486200"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2389"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/bies.950180604"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.2174\/138920305774933286"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M111.009233"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0021199"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0008835"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00504.2009"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.12.161"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj20031163"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.252718"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20121751"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-8628(19980428)77:1<31::AID-AJMG8>3.0.CO;2-O"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1971.tb01651.x"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000013290.12884.A3"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00048.2003"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm763"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1185\/030079904125003143"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2005.12.002"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1186\/ar573"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2807"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2989"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2006.06.079"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M407186200"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1204991109"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2127"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2010.202"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-012-1106-2"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2125"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.14.5578"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.271.5247.360"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2370923"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.13.4428-4438.2003"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122.11.3587"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.4.1221-1230.2003"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00855a019"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650071119"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1994.tb18815.x"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.34.5.400"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(89)90335-5"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)50393-3"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2004.23406.x"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)82320-1"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.32213"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20084"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1305464110","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T22:18:18Z","timestamp":1649801898000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1305464110"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8,12]]},"references-count":53,"journal-issue":{"issue":"35","published-print":{"date-parts":[[2013,8,27]]}},"alternative-id":["10.1073\/pnas.1305464110"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1305464110","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8,12]]},"assertion":[{"value":"2013-08-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}